ArticleToxicological sciences : an official journal of the Society of Toxicology2010
Detection of adduct on tyrosine 411 of albumin in humans poisoned by dichlorvos.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Review: Organophosphorus toxicants, in addition to inhibiting acetylcholinesterase activity, make covalent adducts on multiple proteins and promote protein crosslinking into high molecular weight aggregates.Chemico-biological interactions · 2023Review
- Retrospective detection for V-type OPNAs exposure via phosphonylation and disulfide adducts in albumin.Scientific reports · 2022Article
- Screening of monoclonal antibodies against specific phosphonylation sites and analysis of serum samples exposed to soman and VX using an indirect competitive enzyme-linked immunosorbent assay.Analytical and bioanalytical chemistry · 2022Article
- Organophosphorus pesticide exposure biomarkers in a Mexican population.Environmental science and pollution research international · 2021Article
- The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin.Antioxidants (Basel, Switzerland) · 2020Review
- Monoclonal Antibody That Recognizes Diethoxyphosphotyrosine-Modified Proteins and Peptides Independent of Surrounding Amino Acids.Chemical research in toxicology · 2017Article
- Immunopurification of Acetylcholinesterase from Red Blood Cells for Detection of Nerve Agent Exposure.Chemical research in toxicology · 2017Article
- Biomonitoring Human Albumin Adducts: The Past, the Present, and the Future.Chemical research in toxicology · 2017Article
- An enhanced butyrylcholinesterase method to measure organophosphorus nerve agent exposure in humans.Analytical and bioanalytical chemistry · 2014Article
- PHOS-select iron affinity beads enrich peptides for the detection of organophosphorus adducts on albumin.Chemical research in toxicology · 2013Article
- Protein tyrosine adduct in humans self-poisoned by chlorpyrifos.Toxicology and applied pharmacology · 2013Article
- Review
- Polyclonal antibody to soman-tyrosine.Chemical research in toxicology · 2013Article
- Immunodetection of serum albumin adducts as biomarkers for organophosphorus exposure.The Journal of pharmacology and experimental therapeutics · 2013Article
- Serum creatine phosphokinase: a probable marker of severity in organophosphorus poisoning.Toxicology international · 2011Article
- Reaction of cresyl saligenin phosphate, the organophosphorus agent implicated in aerotoxic syndrome, with human cholinesterases: mechanistic studies employing kinetics, mass spectrometry, and X-ray structure analysis.Chemical research in toxicology · 2011Article
- Development of diagnostics in the search for an explanation of aerotoxic syndrome.Analytical biochemistry · 2010Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Studies in mice and guinea pigs have shown that albumin is a new biomarker of organophosphorus toxicant (OP) and nerve agent exposure. Our goal was to determine whether OP-labeled albumin could be detected in the blood of humans exposed to OP. Blood from four OP-exposed patients was prepared for mass spectrometry analysis by digesting 0.010 ml of serum with pepsin and purifying the labeled albumin peptide by offline high performance liquid chromatography. Dimethoxyphosphate-labeled tyrosine 411 was identified in albumin peptides VRY(411)TKKVPQVSTPTL and LVRY(411)TKKVPQVSTPTL from two patients who had attempted suicide with dichlorvos. The butyrylcholinesterase activity in these serum samples was inhibited 80%. A third patient whose serum BChE activity was inhibited 8% by accidental inhalation of dichlorvos had undetectable levels of adduct on albumin. A fourth patient whose BChE activity was inhibited 60% by exposure to chlorpyrifos had no detectable adduct on albumin. This is the first report to demonstrate the presence of OP-labeled albumin in human patients. It is concluded that tyrosine 411 of human albumin is covalently modified in the serum of humans poisoned by dichlorvos and that the modification is detectable by mass spectrometry. The special reactivity of tyrosine 411 with OP suggests that other proteins may also be modified on tyrosine. Identification of other OP-modified proteins may lead to an understanding of neurotoxic symptoms that appear long after the initial OP exposure.
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