ArticleJournal of structural biology2010
High-throughput expression and purification of membrane proteins.
Article in Journal of structural biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Mechanistic basis of antimicrobial resistance mediated by the phosphoethanolamine transferase MCR-1.Nature communications · 2025Article
- Advancing drug discovery through assay development: a survey of tool compounds within the human solute carrier superfamily.Frontiers in pharmacology · 2024Article
- Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex.Nature communications · 2023Article
- Article
- In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Influence of Detergent and Lipid Composition on Reconstituted Membrane Proteins for Structural Studies.ACS omega · 2021Article
- Production of Recombinant Transmembrane Proteins from Mammalian Cells for Biochemical and Structural Analyses.Current protocols in cell biology · 2020Article
- Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria.Molecular cell · 2020Article
- Spherical-supported membranes as platforms for screening against membrane protein targets.Analytical biochemistry · 2018Article
- Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation.Science (New York, N.Y.) · 2016Article
- Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB and insights into the mechanism of catalysis.Nature communications · 2016Article
- Escherichia coli as host for membrane protein structure determination: a global analysis.Scientific reports · 2015Article
- Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.Biochemistry · 2015Article
- High-throughput cloning and expression of integral membrane proteins in Escherichia coli.Current protocols in protein science · 2013Article
- A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.Lab on a chip · 2013Article
- The crystal structure of α-Dioxygenase provides insight into diversity in the cyclooxygenase-peroxidase superfamily.Biochemistry · 2013Article
- Overexpression of membrane proteins in mammalian cells for structural studies.Molecular membrane biology · 2013Review
- High throughput platforms for structural genomics of integral membrane proteins.Current opinion in structural biology · 2011Review
- Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield.Microbial cell factories · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
High-throughput (HT) methodologies have had a tremendous impact on structural biology of soluble proteins. High-resolution structure determination relies on the ability of the macromolecule to form ordered crystals that diffract X-rays. While crystallization remains somewhat empirical, for a given protein, success is proportional to the number of conditions screened and to the number of variants trialed. HT techniques have greatly increased the number of targets that can be trialed and the rate at which these can be produced. In terms of number of structures solved, membrane proteins appear to be lagging many years behind their soluble counterparts. Likewise, HT methodologies for production and characterization of these hydrophobic macromolecules are only now emerging. Presented here is an HT platform designed exclusively for membrane proteins that has processed over 5000 targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.