ArticleThe Journal of comparative neurology2010
Identifying the efferent projections of leptin-responsive neurons in the dorsomedial hypothalamus using a novel conditional tracing approach.
Article in The Journal of comparative neurology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 81 citations in OpenAlex.
- Advancements in delivery systems for in vivo chimeric antigen receptor T-cell therapy in urological diseases.Current urology · 2026Review
- BoutonNet: an automatic method to detect anterogradely labeled presynaptic boutons in brain tissue sections.Brain structure & function · 2022Article
- The physiological control of eating: signals, neurons, and networks.Physiological reviews · 2022 · on this mapArticle
- Sympathetic innervation of inguinal white adipose tissue in the mouse.The Journal of comparative neurology · 2021Article
- Article
- Ablation of olfactory bulb glutamatergic neurons induces depressive-like behaviors and sleep disturbances in mice.Psychopharmacology · 2020Article
- Anatomical Markers of Activity in Hypothalamic Neurons.Comprehensive Physiology · 2020Review
- Incendiary Leptin.Nutrients · 2020Review
- Neural connectivity between the hypothalamic supramammillary nucleus and appetite- and motivation-related regions of the rat brain.Journal of neuroendocrinology · 2020Article
- Low-dose infusions of leptin into the nucleus of the solitary tract increase sensitivity to third ventricle leptin.American journal of physiology. Endocrinology and metabolism · 2019Article
- Cell-Type Identification in the Autonomic Nervous System.Neuroscience bulletin · 2019Review
- Leptin and brain-adipose crosstalks.Nature reviews. Neuroscience · 2018Review
- A role for leptin-regulated neurocircuitry in subordination stress.Physiology & behavior · 2017Review
- Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.The Journal of comparative neurology · 2017Article
- Hypothalamic growth hormone receptor (GHR) controls hepatic glucose production in nutrient-sensing leptin receptor (LepRb) expressing neurons.Molecular metabolism · 2017Article
- Descending projections from the basal forebrain to the orexin neurons in mice.The Journal of comparative neurology · 2017Article
- Emerging Signaling Pathway in Arcuate Feeding-Related Neurons: Role of the Acbd7.Frontiers in neuroscience · 2017Review
- Lipopolysacharide Rapidly and Completely Suppresses AgRP Neuron-Mediated Food Intake in Male Mice.Endocrinology · 2016Article
- PPARγ mRNA in the adult mouse hypothalamus: distribution and regulation in response to dietary challenges.Frontiers in neuroanatomy · 2015Article
- Whole-brain mapping of the direct inputs and axonal projections of POMC and AgRP neurons.Frontiers in neuroanatomy · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Tracing the axonal projections of selected neurons is labor intensive and inherently limited by currently available neuroanatomical methods. We developed an adeno-associated virus (AAV) that can be used for efficiently tracing identified neuronal populations. The virus encodes a humanized Renilla green fluorescent protein (hrGFP) that is transcriptionally silenced by a neo cassette flanked by LoxH/LoxP sites (AAV-lox-Stop-hrGFP). Thus, hrGFP is expressed only in neurons with Cre recombinase activity. To demonstrate the utility of this approach, the virus was injected unilaterally into the dorsomedial hypothalamus (DMH) of mice that express Cre in neurons expressing the leptin receptor. Animals with DMH injections showed robust hrGFP expression in DMH neurons, as visualized by its endogenous fluorescence or following immunolabeling. We found that hrGFP was expressed in approximately one-third to one-half of Cre-expressing neurons at the site of injection, but not in non-Cre-expressing neurons. The expression of GFP allowed us to identify the projection fields of DMH leptin-responsive neurons. Our results show hrGFP-positive axonal projections and terminals in the paraventricular nucleus of the hypothalamus, arcuate nucleus, preoptic area, bed nucleus of the stria terminalis, paraventricular thalamus, periaqueductal gray, and precoeruleus. The aforementioned pattern of projections was similar to DMH projections determined by injections of biotinylated dextran amine in the mouse DMH. Interestingly, some hrGFP-positive terminals were seen contacting the ependymal layer of the third and fourth ventricles. In summary, this approach is an effective tool for tracing axonal projections of chemically identified neurons, including leptin-responsive neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.