ArticleInvestigative ophthalmology & visual science2010
Retinal sphingolipids and their very-long-chain fatty acid-containing species.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
- Article
- Dysregulated serum lipid profiles in neovascular age-related macular degeneration revealed by UPLC‒MS/MS lipidomics.Lipids in health and disease · 2025Article
- Elevated Very-Long-Chain Ceramides in the Vitreous Humor of Patients With Proliferative Diabetic Retinopathy.Investigative ophthalmology & visual science · 2025Article
- Lipid profile in the aqueous humor of diabetic macular edema patients.Frontiers in medicine · 2025Article
- Review of Eukaryote Cellular Membrane Lipid Composition, with Special Attention to the Fatty Acids.International journal of molecular sciences · 2023Review
- The role of sphingolipids in meibomian gland dysfunction and ocular surface inflammation.The ocular surface · 2022Review
- Profiling Phospholipids within Atlantic SalmonMetabolites · 2022Article
- Dynamic lipid turnover in photoreceptors and retinal pigment epithelium throughout life.Progress in retinal and eye research · 2022Review
- W246G Mutant ELOVL4 Impairs Synaptic Plasticity in Parallel and Climbing Fibers and Causes Motor Defects in a Rat Model of SCA34.Molecular neurobiology · 2021Article
- A look into retinal organoids: methods, analytical techniques, and applications.Cellular and molecular life sciences : CMLS · 2021Review
- Absence of retbindin blocks glycolytic flux, disrupts metabolic homeostasis, and leads to photoreceptor degeneration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Elovl4 Spinocerebellar Ataxia-34 Mutation 736T>G (p.W246G) Impairs Retinal Function in the Absence of Photoreceptor Degeneration.Molecular neurobiology · 2020Article
- Recent advances in the mass spectrometric analysis of glycosphingolipidome - A review.Analytica chimica acta · 2020Review
- Characterization of the phenotype with cognitive impairment and protein mislocalization in SCA34.Neurology. Genetics · 2020Article
- Review
- Decreased very long chain polyunsaturated fatty acids in sperm correlates with sperm quantity and quality.Journal of assisted reproduction and genetics · 2019Article
- Clinical signs of meibomian gland dysfunction (MGD) are associated with changes in meibum sphingolipid composition.The ocular surface · 2019Article
- Conditional deletion of Des1 in the mouse retina does not impair the visual cycle in cones.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- ELOVL4: Very long-chain fatty acids serve an eclectic role in mammalian health and function.Progress in retinal and eye research · 2019Review
- Sphingolipids as Emerging Mediators in Retina Degeneration.Frontiers in cellular neuroscience · 2019Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeRecent evidence suggests that ceramide metabolism plays an important role in retinal photoreceptor cell survival and apoptosis. The purpose of this study was to characterize sphingolipids in the retina with special emphasis on the very-long-chain-containing saturated (VLC-FA) and polyunsaturated (VLC-PUFA) fatty acid-containing species. The VLC-FAs and VLC-PUFAs are synthesized by the ELOVL4 protein, which is involved in human Stargardt's macular dystrophy type 3 (STGD3).
methodsTotal lipids were extracted from retina and other tissues, and different sphingolipid classes were isolated and purified using various combinations of liquid- and solid-phase separation. Purified sphingolipids were analyzed by high-performance thin layer chromatography (HPTLC), gas chromatography (GC), and GC-MS (GC-mass spectrometry).
resultsNonsialylated sphingolipids (NSLs) comprised approximately 3.5% of total retinal lipids of which 70% was sphingomyelin. Ceramide and glycosylceramides (GCs) constituted<or=1% of total retinal lipids. Gangliosides (GGs), on the other hand, comprised approximately 3.0% of total retinal lipids. Fatty acid analysis of retinal NSLs indicated an abundance of saturated fatty acids, with the presence of VLC-FAs but not of VLC-PUFAs beyond 24 carbons. However, GG had significant levels of unsaturated, polyunsaturated, and VLC-PUFAs. Retinal rod outer segments (ROS) contained approximately 1% each of NSL and GG, and their fatty acid profile was not very different from whole retinal NSL and GG, respectively.
conclusionsRetina has a total of 6% to 7% fatty acids that are N-linked to a sphingosine, which would be 11 to 13 mole % in comparison to phospholipids. The presence of VLC-FAs and VLC-PUFAs in retinal sphingolipids indicates that they may play role in ELOVL4-mediated Stargardt 3.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.