ArticleInvestigative ophthalmology & visual science2010
Identification of the neuromuscular junction transcriptome of extraocular muscle by laser capture microdissection.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- The Neuromuscular Junction: A Shared Vulnerability in Aging and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Review
- Developmental, Physiological and Phylogenetic Perspectives on the Expression and Regulation of Myosin Heavy Chains in Craniofacial Muscles.International journal of molecular sciences · 2024Review
- Normobaric hypoxia shows enhanced FOXO1 signaling in obese mouse gastrocnemius muscle linked to metabolism and muscle structure and neuromuscular innervation.Pflugers Archiv : European journal of physiology · 2023Article
- Looking for answers far away from the soma-the (un)known axonal functions of TDP-43, and their contribution to early NMJ disruption in ALS.Molecular neurodegeneration · 2023Review
- Models and methods to study Schwann cells.Journal of anatomy · 2022Review
- Misfolding at the synapse: A role in amyotrophic lateral sclerosis pathogenesis?Frontiers in molecular neuroscience · 2022Review
- Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers.Nature communications · 2020Article
- Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle.Frontiers in molecular neuroscience · 2020Article
- Extraocular muscle function is impaired inThe Journal of general physiology · 2019Article
- R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5.Scientific reports · 2016Article
- Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.Proteome science · 2014Article
- Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.Skeletal muscle · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo examine and characterize the profile of genes expressed at the synapses or neuromuscular junctions (NMJs) of extraocular muscles (EOMs) compared with those expressed at the tibialis anterior (TA).
methodsAdult rat eyeballs with rectus EOMs attached and TAs were dissected, snap frozen, serially sectioned, and stained for acetylcholinesterase (AChE) to identify the NMJs. Approximately 6000 NMJs for rectus EOM (EOMsyn), 6000 NMJs for TA (TAsyn), equal amounts of NMJ-free fiber regions (EOMfib, TAfib), and underlying myonuclei and RNAs were captured by laser capture microdissection (LCM). RNA was processed for microarray-based expression profiling. Expression profiles and interaction lists were generated for genes differentially expressed at synaptic and nonsynaptic regions of EOM (EOMsyn versus EOMfib) and TA (TAsyn versus TAfib). Profiles were validated by using real-time quantitative polymerase chain reaction (qPCR).
resultsThe regional transcriptomes associated with NMJs of EOMs and TAs were identified. Two hundred seventy-five genes were preferentially expressed in EOMsyn (compared with EOMfib), 230 in TAsyn (compared with TAfib), and 288 additional transcripts expressed in both synapses. Identified genes included novel genes as well as well-known, evolutionarily conserved synaptic markers (e.g., nicotinic acetylcholine receptor (AChR) alpha (Chrna) and epsilon (Chrne) subunits and nestin (Nes).
conclusionsTranscriptome level differences exist between EOM synaptic regions and TA synaptic regions. The definition of the synaptic transcriptome provides insight into the mechanism of formation and functioning of the unique synapses of EOM and their differential involvement in diseases noted in the EOM allotype.
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