ArticleClinical and vaccine immunology : CVI2010
Rapid, simple, quantitative, and highly sensitive antibody detection for lyme disease.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research.PloS one · 2016Pooled it
- Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes.Scientific reports · 2017Trial
- The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease.Journal of clinical microbiology · 2025Article
- Quantitative multiplexed strategies for human Lyme disease serological testing.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Lethal Hemorrhagic Disease and Clinical Illness Associated with Elephant Endotheliotropic Herpesvirus 1 Are Caused by Primary Infection: Implications for the Detection of Diagnostic Proteins.Journal of virology · 2020Article
- Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Revisiting the Lyme Disease Serodiagnostic Algorithm: the Momentum Gathers.Journal of clinical microbiology · 2018Article
- Article
- Vaccination against Lyme disease: Are we ready for it?Human vaccines & immunotherapeutics · 2016Article
- Commercial test kits for detection of Lyme borreliosis: a meta-analysis of test accuracy.International journal of general medicine · 2016Article
- Evaluation of Selected Borrelia burgdorferi lp54 Plasmid-Encoded Gene Products Expressed during Mammalian Infection as Antigens To Improve Serodiagnostic Testing for Early Lyme Disease.Clinical and vaccine immunology : CVI · 2015Article
- Laboratory diagnosis of Lyme disease: advances and challenges.Infectious disease clinics of North America · 2015Review
- Luciferase immunoprecipitation systems for measuring antibodies in autoimmune and infectious diseases.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Article
- C6 Peptide-Based Multiplex Phosphorescence Analysis (PHOSPHAN) for Serologic Confirmation of Lyme Borreliosis.PloS one · 2015Article
- Simple objective detection of human lyme disease infection using immuno-PCR and a single recombinant hybrid antigen.Clinical and vaccine immunology : CVI · 2014Article
- Identification of OppA2 linear epitopes as serodiagnostic markers for Lyme disease.Clinical and vaccine immunology : CVI · 2014Article
- Simple and sensitive detection of HBsAg by using a quantum dots nanobeads based dot-blot immunoassay.Theranostics · 2014Article
- Lack of serum antibodies against Borrelia burgdorferi in children with autism.Clinical and vaccine immunology : CVI · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
There is currently a need for improved serological tests for the diagnosis and monitoring of Lyme disease, an infection caused by Borrelia burgdorferi. In the present study, we evaluated luciferase immunoprecipitation systems (LIPSs) for use for profiling of the antibody responses to a panel of B. burgdorferi proteins for the diagnosis of Lyme disease. Initially, serum samples from a cohort of patients and controls (n = 46) were used for training and were profiled by the use of 15 different B. burgdorferi antigen constructs. For the patient sera, the antibody responses to several B. burgdorferi antigens, including VlsE, flagellin (FlaB), BmpA, DbpA, and DbpB, indicated that the antigens had high levels of immunoreactivity. However, the best diagnostic performance was achieved with a synthetic protein, designated VOVO, consisting of a repeated antigenic peptide sequence, VlsE-OspC-VlsE-OspC, Analysis of an independent set of serum samples (n = 139) used for validation showed that the VOVO LIPS test had 98% sensitivity (95% confidence interval [CI], 93% to 100%; P < 0.0001) and 100% specificity (95% CI, 94% to 100%; P < 0.0001). Similarly, the C6 peptide enzyme-linked immunosorbent assay (ELISA) also had 98% sensitivity (95% CI, 93% to 100%; P < 0.0001) and 98% specificity (95% CI, 90% to 100%; P < 0.0001). Receiver operating characteristic analysis revealed that the rates of detection of Lyme disease by the LIPS test and the C6 ELISA were not statistically different. However, the VOVO LIPS test displayed a wide dynamic range of antibody detection spanning over 10,000-fold without the need for serum dilution. These results suggest that screening by the LIPS test with VOVO and other B. burgdorferi antigens offers an efficient quantitative approach for evaluation of the antibody responses in patients with Lyme disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.