Evidence map›Paper›PMID 20390134›Full record

ArticleLab on a chip2010

DNA sequencing by denaturation: experimental proof of concept with an integrated fluidic device.

Ying-Ja Chen, Eric E Roller, Xiaohua Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ying-Ja ChenDepartment of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA. x2huang@ucsd.edu
Eric E Roller
Xiaohua Huang

Funding

Direct real-time single molecule DNA sequencingR21HG005096 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HUANG, XIAOHUA · 2010 to 2012
$1.1M
Massively Parallel Cloning and Sequencing of DNAR21HG003587 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HUANG, XIAOHUA · 2005 to 2007
$731k
Genome Sequencing by Ligation Using Nano-Arrays of Single DNA MoleculesR21HG004130 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HUANG, XIAOHUA · 2006 to 2007
$501k
NHGRI NIH HHS 1R01HG005096NHGRI NIH HHS R21 HG003587NHGRI NIH HHS R21HG003587NHGRI NIH HHS R21 HG004130NHGRI NIH HHS R21HG004130NHGRI NIH HHS R21 HG005096NHGRI NIH HHS R33 HG004130
6 · The paper itself

Abstract

We report the proof of concept of a novel DNA sequencing technology called sequencing by denaturation (SBD). SBD is based on the Sanger sequencing reaction performed on amplified target templates immobilized on a solid surface followed by the denaturation of these Sanger fragments. As these fluorescently labeled fragments denature sequentially, the fluorescence intensities in the four channels corresponding to the four base types are monitored in a flow cell. A sequencing instrument with a microfluidic flowcell has been custom-designed to integrate automated fluidics, temperature control, and fluorescence imaging. The denaturation profiles of several synthetic oligonucleotides were measured with this system and our data demonstrated the ability to sequence short DNA by SBD. SBD is a simple and fast method with the potential to improve the speed and cost of large-scale genome re-sequencing.

Indexed as

Base SequenceDNAEquipment DesignEquipment Failure AnalysisMicrofluidic Analytical TechniquesMolecular Sequence DataNucleic Acid DenaturationPilot ProjectsSequence Analysis, DNASystems IntegrationDNA

Identifiers

PMID20390134
PMCPMC2881221

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.