ArticleOptics express2010
A general method for improving spatial resolution by optimization of electron multiplication in CCD imaging.
Article in Optics express, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Detecting and measuring of GPCR signaling - comparison of human induced pluripotent stem cells and immortal cell lines.Frontiers in endocrinology · 2023Review
- Single-molecule counting applied to the study of GPCR oligomerization.Biophysical journal · 2022Review
- Optimizing Long-Term Live Cell Imaging.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Decomposition of cell activities revealing the role of the cell cycle in driving biofunctional heterogeneity.Scientific reports · 2021Article
- Functional interplay between the cell cycle and cell phenotypes.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Fisher information matrix for branching processes with application to electron-multiplying charge-coupled devices.Multidimensional systems and signal processing · 2012Article
- High-throughput ballistic injection nanorheology to measure cell mechanics.Nature protocols · 2012Article
- Cell cycle-dependent alteration in NAC1 nuclear body dynamics and morphology.Physical biology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The electron-multiplying charge-coupled device (EMCCD) camera possesses an electron multiplying function that can effectively convert the weak incident photon signal to amplified electron output, thereby greatly enhancing the contrast of the acquired images. This device has become a popular photon detector in single-cell biophysical assays to enhance subcellular images. However, the quantitative relationship between the resolution in such measurements and the electron multiplication setting in the EMCCD camera is not well-understood. We therefore developed a method to characterize the exact dependence of the signal-to-noise-ratio (SNR) on EM gain settings over a full range of incident light intensity. This information was further used to evaluate the EMCCD performance in subcellular particle tracking. We conclude that there are optimal EM gain settings for achieving the best SNR and the best spatial resolution in these experiments. If it is not used optimally, electron multiplication can decrease the SNR and increases spatial error.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.