ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
Low Current-driven Micro-electroporation Allows Efficient In Vivo Delivery of Nonviral DNA into the Adult Mouse Brain.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Electroporation of Whole-Mount Postnatal Rodent Retinas for Advanced Functional Assays.Bio-protocol · 2026Article
- Gene Electrotransfer via Conductivity-Clamped Electric Field Focusing Pivots Sensori-Motor DNA Therapeutics: "A Spoonful of Sugar Helps the Medicine Go Down".Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Hydrogen Sulfide Protects against Rat Ischemic Brain Injury by Promoting RhoA Phosphorylation at Serine 188.ACS omega · 2024Article
- Optimization of piggyBac Transposon System Electrotransfection in Sheep Fibroblasts.Molecular biotechnology · 2023Article
- Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders.International journal of molecular sciences · 2023Review
- Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model.Biomedicines · 2022Article
- Direct Reprogramming of Somatic Cells to Neurons: Pros and Cons of Chemical Approach.Neurochemical research · 2021Article
- Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts.BMC molecular and cell biology · 2020Article
- Non-viral Gene Delivery Methods for Bone and Joints.Frontiers in bioengineering and biotechnology · 2020Review
- A 3D Printed Device for Low Cost Neural Stimulation in Mice.Frontiers in neuroscience · 2019Article
- Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Rapid and efficient gene delivery into the adult mouse brain via focal electroporation.Scientific reports · 2016Article
- In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation.Nature protocols · 2015Article
- The impact of non-electrical factors on electrical gene transfer.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Voltage Preconditioning Allows Modulated Gene Expression in Neurons Using PEI-complexed siRNA.Molecular therapy. Nucleic acids · 2013Article
- Article
- Muscle gene electrotransfer is increased by the antioxidant tempol in mice.Gene therapy · 2012Article
- The role of pH fronts in reversible electroporation.PloS one · 2011Article
- Second Harmonic Generation for time-resolved monitoring of membrane pore dynamics subserving electroporation of neurons.Biomedical optics express · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viral gene transfer or transgenic animals are commonly used technologies to alter gene expression in the adult brain, although these approaches lack spatial specificity and are time consuming. We delivered plasmid DNA locally into the brain of adult C57BL/6 mice in vivo by voltage- and current-controlled electroporation. The low current-controlled delivery of unipolar square wave pulses of 125 µA with microstimulation electrodes at the injection site gave 16 times higher transfection rates than a voltage-controlled electroporation protocol with plate electrodes resulting in currents of about 400 mA. Transfection was restricted to the target region and no damage due to the electric pulses was found. Our current-controlled electroporation protocol indicated that the use of very low currents resulting in applied voltages within the physiological range of the membrane potential, allows efficient transfection of nonviral plasmid DNA. In conclusion, low current-controlled electroporation is an excellent approach for electroporation in the adult brain, i.e., gene function can be influenced locally at a high level with no mortality and minimal tissue damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.