ArticleThe EMBO journal2010
An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Phosphorylated BLM peptide acts as an agonist for DNA damage response.Nucleic acids research · 2025Article
- DDX41 is required for cGAS-STING activation against DNA virus infection.Cell reports · 2022Article
- Review
- Bloom's Syndrome: Clinical Spectrum, Molecular Pathogenesis, and Cancer Predisposition.Molecular syndromology · 2017Review
- Bloom's syndrome: Why not premature aging?: A comparison of the BLM and WRN helicases.Ageing research reviews · 2017Review
- A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-End.Journal of molecular biology · 2016Article
- Multimerization domains are associated with apparent strand exchange activity in BLM and WRN DNA helicases.DNA repair · 2014Article
- The role of post-translational modifications in fine-tuning BLM helicase function during DNA repair.DNA repair · 2014Review
- The dissolution of double Holliday junctions.Cold Spring Harbor perspectives in biology · 2014Review
- Smc5/6-Mms21 prevents and eliminates inappropriate recombination intermediates in meiosis.PLoS genetics · 2013Article
- Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes.PLoS genetics · 2013Article
- BLM SUMOylation regulates ssDNA accumulation at stalled replication forks.Frontiers in genetics · 2013Article
- RAPADILINO RECQL4 mutant protein lacks helicase and ATPase activity.Biochimica et biophysica acta · 2012Article
- Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes.BMC molecular biology · 2012Article
- Synergic and opposing activities of thermophilic RecQ-like helicase and topoisomerase 3 proteins in Holliday junction processing and replication fork stabilization.The Journal of biological chemistry · 2012Article
- Functional analyses of human DNA repair proteins important for aging and genomic stability using yeast genetics.DNA repair · 2012Review
- Unwinding and rewinding: double faces of helicase?Journal of nucleic acids · 2012Article
- Critical interaction domains between bloom syndrome protein and RAD51.The protein journal · 2011Article
- RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana.The Plant cell · 2010Article
- Rif1 provides a new DNA-binding interface for the Bloom syndrome complex to maintain normal replication.The EMBO journal · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The gene mutated in Bloom's syndrome, BLM, encodes a member of the RecQ family of DNA helicases that is needed to suppress genome instability and cancer predisposition. BLM is highly conserved and all BLM orthologs, including budding yeast Sgs1, have a large N-terminus that binds Top3-Rmi1 but has no known catalytic activity. In this study, we describe a sub-domain of the Sgs1 N-terminus that shows in vitro single-strand DNA (ssDNA) binding, ssDNA annealing and strand-exchange (SE) activities. These activities are conserved in the human and Drosophila orthologs. SE between duplex DNA and homologous ssDNA requires no cofactors and is inhibited by a single mismatched base pair. The SE domain of Sgs1 is required in vivo for the suppression of hyper-recombination, suppression of synthetic lethality and heteroduplex rejection. The top3Delta slow-growth phenotype is also SE dependent. Surprisingly, the highly divergent human SE domain functions in yeast. This work identifies SE as a new molecular function of BLM/Sgs1, and we propose that at least one role of SE is to mediate the strand-passage events catalysed by Top3-Rmi1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.