ArticleThe Journal of biological chemistry2010
Adenosine 2B receptor expression is post-transcriptionally regulated by microRNA.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Restoration of miR-1228* expression suppresses epithelial-mesenchymal transition in gastric cancer.PloS one · 2013Trial
- Article
- Eukaryotic initiation factor 5A hypusine as a negative regulator of adenosine 2B receptor (A2bAR) through interaction with stem loop sequences within the A2bAR 3'-untranslated region.Molecular biology reports · 2023Article
- MicroRNA: Crucial modulator in purinergic signalling involved diseases.Purinergic signalling · 2023Review
- The Purinergic System as a Pharmacological Target for the Treatment of Immune-Mediated Inflammatory Diseases.Pharmacological reviews · 2019Review
- MicroRNA in gastrointestinal cell signalling.Inflammopharmacology · 2018Review
- Role of G protein-coupled receptors-microRNA interactions in gastrointestinal pathophysiology.American journal of physiology. Gastrointestinal and liver physiology · 2017Review
- Lung injury pathways: Adenosine receptor 2B signaling limits development of ischemic bronchiolitis obliterans organizing pneumonia.Experimental lung research · 2017Article
- MicroRNA-16 is putatively involved in the NF-κB pathway regulation in ulcerative colitis through adenosine A2a receptor (A2aAR) mRNA targeting.Scientific reports · 2016Article
- Computational prediction of candidate miRNAs and their potential functions in biomineralization in pearl oyster Pinctada martensii.Saudi journal of biological sciences · 2016Article
- Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis.Current genomics · 2015Article
- Regulation of IRS1/Akt insulin signaling by microRNA-128a during myogenesis.Journal of cell science · 2013Article
- Regulation of miR106b cluster through the RB pathway: mechanism and functional targets.Cell cycle (Georgetown, Tex.) · 2013Article
- Chromatin state and microRNA determine different gene expression dynamics responsive to TNF stimulation.Genomics · 2012Article
- Contributions of A2A and A2B adenosine receptors in coronary flow responses in relation to the KATP channel using A2B and A2A/2B double-knockout mice.American journal of physiology. Heart and circulatory physiology · 2011Article
- Role for microRNAs in regulating glucocorticoid response and resistance in multiple myeloma.Hormones & cancer · 2011Article
- The resurgence of A2B adenosine receptor signaling.Biochimica et biophysica acta · 2011Review
- Distinct roles for the A2B adenosine receptor in acute and chronic stages of bleomycin-induced lung injury.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Tumour necrosis factor-α (TNF-α) and miRNA expression in frontal and temporal neocortex in Alzheimer's disease and the effect of TNF-α on miRNA expression in vitro.International journal of molecular epidemiology and genetics · 2011Article
- Role of adenosine A(2B) receptors in inflammation.Advances in pharmacology (San Diego, Calif.) · 2011Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
We have reported that epithelial adenosine 2B receptor (A(2B)AR) mRNA and protein are up-regulated in colitis, which we demonstrated to be regulated by tumor necrosis factor alpha (TNF-alpha). Here, we examined the mechanism that governs A(2B)AR expression during colitis. A 1.4-kb sequence of the A(2B)AR promoter was cloned into the pFRL7 luciferase vector. Anti-microRNA (miRNA) was custom-synthesized based on specific miRNA binding sites. The binding of miRNA to the 3'-untranslated region (UTR) of A(2B)AR mRNA was examined by cloning this 3'-UTR downstream of the luciferase gene in pMIR-REPORT. In T84 cells, TNF-alpha induced a 35-fold increase in A(2B)AR mRNA but did not increase promoter activity in luciferase assays. By nuclear run-on assay, no increase in A(2B)AR mRNA following TNF-alpha treatment was observed. Four putative miRNA target sites (miR27a, miR27b, miR128a, miR128b) in the 3'-UTR of the A(2B)AR mRNA were identified in T84 cells and mouse colon. Pretreatment of cells with TNF-alpha reduced the levels of miR27b and miR128a by 60%. Over expression of pre-miR27b and pre-miR128a reduced A(2B)AR levels by >60%. Blockade of miR27b increased A(2B)AR mRNA levels by 6-fold in vitro. miR27b levels declined significantly in colitis-affected tissue in mice in the presence of increased A(2B)AR mRNA. Collectively, these data demonstrate that TNF-alpha-induced A(2B)AR expression in colonic epithelial cells is post-transcriptionally regulated by miR27b and miR128a and show that miR27b influences A(2B)AR expression in murine colitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.