ArticlePlant physiology2010
Virus-based microRNA expression for gene functional analysis in plants.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 138 citations in OpenAlex.
- A transposon insertion in the promoter ofHorticulture research · 2026Article
- Abscisic acid-regulated stability of CmABF1 and CmBRM modulates salt tolerance in chrysanthemum via epigenetic regulation ofScience advances · 2026Article
- Alternaria alternata Effector Aa593 Promotes Virulence by Hijacking the CmNAC29-Mediated Abscisic Acid Biosynthesis Pathway in Chrysanthemum.Plant biotechnology journal · 2026Article
- APathogens (Basel, Switzerland) · 2025Article
- Gene silencing in plants by artificial small RNAs derived from minimal precursors and expressed via tobacco rattle virus.Plant molecular biology · 2025Article
- Genome-wide identification of BBX gene family and its function in defense of necrotrophic fungus Alternaria sp. in Chrysanthemum.BMC genomics · 2025Article
- Salicylic acid regulates biosynthesis of floral fragrance (E)-β-farnesene via NPR3-WRKY1 module in chrysanthemum.Molecular horticulture · 2025Article
- Article
- CmHRE2L-CmACS6 transcriptional cascade negatively regulates waterlogging tolerance in Chrysanthemum.Molecular horticulture · 2025Article
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- The plant siRNA landscape.The Plant cell · 2024Review
- Applications of Virus-Induced Gene Silencing in Cotton.Plants (Basel, Switzerland) · 2024Review
- PHOTOLYASE/BLUE LIGHT RECEPTOR2 regulates chrysanthemum flowering by compensating for gibberellin perception.Plant physiology · 2023Article
- Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors.Nucleic acids research · 2023Article
- Virus-Induced Gene Silencing in the Tea Plant (Plants (Basel, Switzerland) · 2023Article
- vsiRNA18 derived from tobacco curly shoot virus can regulate virus infection in Nicotiana benthamiana.Molecular plant pathology · 2023Article
- Chrysanthemum MAF2 regulates flowering by repressing gibberellin biosynthesis in response to low temperature.The Plant journal : for cell and molecular biology · 2022Article
- Artificial microRNA guide strand selection from duplexes with no mismatches shows a purine-rich preference for virus- and non-virus-based expression vectors in plants.Plant biotechnology journal · 2022Article
- Systemic silencing of an endogenous plant gene by two classes of mobile 21-nucleotide artificial small RNAs.The Plant journal : for cell and molecular biology · 2022Article
- Members of the ribosomal protein S6 (RPS6) family act as pro-viral factor for tomato spotted wilt orthotospovirus infectivity in Nicotiana benthamiana.Molecular plant pathology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditional virus-induced gene silencing (VIGS) is a powerful virus-based short interfering RNA-mediated RNA silencing technique for plant functional genomics. Besides short interfering RNAs, microRNAs (miRNAs) have also been shown to regulate gene expression by RNA silencing in various organisms. However, plant virus-based miRNA silencing has not been reported. In addition, a number of plant miRNAs have been identified or predicted, while their functions are largely unknown. Thus, there is an urgent need for the development of new technologies to study miRNA function. Here, we report that a modified cabbage leaf-curl geminivirus vector can be used to express artificial and endogenous miRNAs in plants. Using this viral miRNA expression system, we demonstrate that VIGS using artificial miRNAs, dubbed as "MIR VIGS," was effective to silence the expression of endogenous genes, including PDS, Su, CLA1, and SGT1, in Nicotiana benthamiana. Silencing of SGT1 led to the loss of N-mediated resistance to Tobacco mosaic virus. Furthermore, using this viral miRNA expression system, we found that viral ectopic expression of endogenous miR156 and miR165 but not their mutants in N. benthamiana resulted in earlier abnormal developmental phenotypes, and expression of miR165 induced abnormal chlorotic spots on leaves. These results demonstrate that the cabbage leaf-curl geminivirus-based miRNA expression system can be utilized not only to specifically silence genes involved in general metabolism and defense but also to investigate the function of endogenous miRNAs in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.