ArticlePlant physiology2010
A pollen-specific RALF from tomato that regulates pollen tube elongation.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 162 citations in OpenAlex.
- Plant Peptides on the Rise: From Historical Insight to Future Applications.Plant biotechnology journal · 2026Review
- Establishment of fluorescent protein-tagged lines for investigating dynamic localization of organelle and other cell structures in rice pollen tube.Plant methods · 2026Article
- The RALF-FERONIA Signaling Axis: A Central Hub Integrating Plant Growth, Reproduction, and Stress Responses.International journal of molecular sciences · 2025Review
- From roots to reproduction: the multifaceted roles of rapid alkalinization factor and epidermal patterning factor peptides in plants.Journal of experimental botany · 2025Review
- Gene expression and pollen performance indicate altered postmating selection between Solanum species with different mating systems.G3 (Bethesda, Md.) · 2025Article
- Peptide hormones in plants.Molecular horticulture · 2025Review
- The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize.The Plant cell · 2024Article
- The PPO family in Nicotiana tabacum is an important regulator to participate in pollination.BMC plant biology · 2024Article
- Deep learning-based high-throughput detection of in vitro germination to assess pollen viability from microscopic images.Journal of experimental botany · 2023Article
- Emerging Roles of Receptor-like Protein Kinases in Plant Response to Abiotic Stresses.International journal of molecular sciences · 2023Review
- Article
- Rapid alkalinization factor: function, regulation, and potential applications in agriculture.Stress biology · 2023Review
- Genome-Wide Identification and Comparative Analysis ofInternational journal of molecular sciences · 2023Article
- Molecular Mechanisms of Regulation of Root Development by Plant Peptides.Plants (Basel, Switzerland) · 2023Review
- TheInternational journal of molecular sciences · 2023Article
- Tomato plant response to heat stress: a focus on candidate genes for yield-related traits.Frontiers in plant science · 2023Review
- Maize Transcription FactorInternational journal of molecular sciences · 2022Article
- Peptides/receptors signaling during plant fertilization.Frontiers in plant science · 2022Review
- Genome-wide characterization of soybeanFrontiers in plant science · 2022Article
- Combined transcriptomic and proteomic analysis reveals multiple pathways involved in self-pollen tube development and the potential roles of FviYABBY1 in self-incompatibility inFrontiers in plant science · 2022Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid Alkalinization Factors (RALFs) are plant peptides that rapidly increase the pH of plant suspension cell culture medium and inhibit root growth. A pollen-specific tomato (Solanum lycopersicum) RALF (SlPRALF) has been identified. The SlPRALF gene encodes a preproprotein that appears to be processed and released from the pollen tube as an active peptide. A synthetic SlPRALF peptide based on the putative active peptide did not affect pollen hydration or viability but inhibited the elongation of normal pollen tubes in an in vitro growth system. Inhibitory effects of SlPRALF were detectable at concentrations as low as 10 nm, and complete inhibition was observed at 1 mum peptide. At least 10-fold higher levels of alkSlPRALF, which lacks disulfide bonds, were required to see similar effects. A greater effect of peptide was observed in low-pH-buffered medium. Inhibition of pollen tube elongation was reversible if peptide was removed within 15 min of exposure. Addition of 100 nm SlPRALF to actively growing pollen tubes inhibited further elongation until tubes were 40 to 60 mum in length, after which pollen tubes became resistant to the peptide. The onset of resistance correlated with the timing of the exit of the male germ unit from the pollen grain into the tube. Thus, exogenous SlPRALF acts as a negative regulator of pollen tube elongation within a specific developmental window.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.