ArticlePlant physiology2010
The changing fate of a secretory glycoprotein in developing maize endosperm.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Imaging the ER and Endomembrane System in Cereal Endosperm.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- Multiscale imaging reveals the presence of autophagic vacuoles in developing maize endosperm.Frontiers in plant science · 2022Article
- Maize Endosperm Development: Tissues, Cells, Molecular Regulation and Grain Quality Improvement.Frontiers in plant science · 2022Review
- Progressive Aggregation of 16 kDa Gamma-Zein during Seed Maturation in TransgenicInternational journal of molecular sciences · 2021Article
- Sweet Modifications Modulate Plant Development.Biomolecules · 2021Review
- Glyco-Engineering of Plant-Based Expression Systems.Advances in biochemical engineering/biotechnology · 2021Article
- Seed-produced anti-globulin VHH-Fc antibodies retrieve globulin precursors in the insoluble fraction and modulate the Arabidopsis thaliana seed subcellular morphology.Plant molecular biology · 2020Article
- Towards coeliac-safe bread.Plant biotechnology journal · 2020Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- 3D Electron Microscopy Gives a Clue: Maize Zein Bodies Bud From Central Areas of ER Sheets.Frontiers in plant science · 2020Article
- Maize 16-kD γ-zein forms very unusual disulfide-bonded polymers in the endoplasmic reticulum: implications for prolamin evolution.Journal of experimental botany · 2018Article
- Article
- Protein accumulation in aleurone cells, sub-aleurone cells and the center starch endosperm of cereals.Plant cell reports · 2014Review
- Fusion, rupture, and degeneration: the fate of in vivo-labelled PSVs in developing barley endosperm.Journal of experimental botany · 2014Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
- Trafficking of endoplasmic reticulum-retained recombinant proteins is unpredictable in Arabidopsis thaliana.Frontiers in plant science · 2014Review
- The Induction of Recombinant Protein Bodies in Different Subcellular Compartments Reveals a Cryptic Plastid-Targeting Signal in the 27-kDa γ-Zein Sequence.Frontiers in bioengineering and biotechnology · 2014Article
- A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and its wild type Mn1 in maize.Frontiers in plant science · 2014Article
- Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins.Plant molecular biology · 2013Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zeins are the major storage proteins in maize (Zea mays) endosperm, and their accumulation in zein bodies derived from the endoplasmic reticulum is well characterized. In contrast, relatively little is known about post-Golgi compartments or the trafficking of vacuolar proteins in maize endosperm, specifically the presence of globulins in structures resembling protein storage vacuoles that appear in early to mid-stage seed development. We investigated this pathway by expressing and analyzing a recombinant reporter glycoprotein during endosperm maturation, using a combination of microscopy and sensitive glycopeptide analysis. Specific N-glycan acceptor sites on the protein were followed through the stages of grain development, revealing a shift from predominantly paucimannosidic vacuolar glycoforms to predominantly trimmed glycan structures lacking fucose. This was accompanied by a change in the main subcellular localization of the protein from large protein storage vacuole-like post-Golgi organelles to the endoplasmic reticulum and zein bodies. The endogenous storage proteins corn alpha-globulin and corn legumin-1 showed a similar spatiotemporal profile both in transgenic plants expressing the reporter glycoprotein and in wild-type plants. This indicates that the shift of the intracellular trafficking route, as observed with our reporter glycoprotein, may be a common strategy in maize seed development.
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Registered trials
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