ArticleBiochemistry2010
Probing the "charge cluster mechanism" in amphipathic helical cationic antimicrobial peptides.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 151 citations in OpenAlex.
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- Structural pores not required: Antimicrobial peptides induce ion permeabilization of lipid membranes through transient water channels.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lipid Shape as a Membrane Activity Modulator of a Fusogenic Antimicrobial Peptide.Journal of chemical information and modeling · 2025Article
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- Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis.Scientific reports · 2023Article
- Stapled β-Hairpin Antimicrobial Peptides with Improved Stability and Activity against Drug-Resistant Gram-Negative Bacteria.Journal of medicinal chemistry · 2023Article
- Deciphering Structure-Function Relationship Unveils Salt-Resistant Mode of Action of a Potent MRSA-Inhibiting Antimicrobial Peptide, RR14.Journal of bacteriology · 2022Article
- An Antimicrobial Peptide-Mimetic Methacrylate Random Copolymer Induces Domain Formation in a Model Bacterial Membrane.The Journal of membrane biology · 2022Review
- Mechanistic Studies of Antibiotic Adjuvants Reducing Kidney's Bacterial Loads upon Systemic Monotherapy.Pharmaceutics · 2021Article
- Insights into the Action Mechanism of the Antimicrobial Peptide Lasioglossin III.International journal of molecular sciences · 2021Article
- Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers.Scientific reports · 2021Article
- Electrostatic effects in saturation of membrane binding of cationic cell-penetrating peptide.European biophysics journal : EBJ · 2021Article
- EcDBS1R4, an Antimicrobial Peptide Effective againstInternational journal of molecular sciences · 2020Article
- Shorter Antibacterial Peptide Having High Selectivity forMicroorganisms · 2020Article
- Analysis of mutations of defensin protein using accelerated molecular dynamics simulations.PloS one · 2020Article
- Localization Preference of Antimicrobial Peptides on Liquid-Disordered Membrane Domains.Frontiers in cell and developmental biology · 2020Article
- The host-defense peptide piscidin P1 reorganizes lipid domains in membranes and decreases activation energies in mechanosensitive ion channels.The Journal of biological chemistry · 2019Article
- Mechanistic insights and therapeutic opportunities of antimicrobial chemokines.Seminars in cell & developmental biology · 2019Review
- Prevention of ESKAPE pathogen biofilm formation by antimicrobial peptides WLBU2 and LL37.International journal of antimicrobial agents · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Clustering of anionic lipids away from zwitterionic ones by cationic antimicrobial agents has recently been established as a mechanism of action of natural small, flexible peptides as well as non-natural synthetic peptide mimics. One of the largest classes of antimicrobial peptides consists of peptides that form cationic amphipathic helices on membranes and whose toxic action is dependent on the formation of pores in the membrane or through the "carpet" mechanism. We have evaluated the role of anionic lipid clustering for five of these peptides, i.e., MSI-78, MSI-103, MSI-469, MSI-843, and MSI-1254, with different sequences and properties. We determined whether these amphipathic helical cationic antimicrobial peptides cluster anionic lipids from zwitterionic ones and if this property is related to the species specificity of their toxicity. All five of these peptides were capable of lipid clustering, in contrast to the well-studied amphipathic helical antimicrobial peptide, magainin 2, which does not. We ascribe this difference to the lower density of positive charges in magainin 2. Peptides that efficiently cluster anionic lipids generally have a ratio of MIC for Staphylococcus aureus to that for Escherichia coli of >1. The addition of an N-terminal octyl chain did not preclude anionic charge clustering, although the ratio of MIC for S. aureus to that for E. coli was somewhat lowered. In most Gram-positive bacteria, there is a predominance of anionic lipids in the cytoplasmic membrane. In Gram-negative bacteria, however, clustering of anionic lipids away from zwitterionic ones is emerging as an important contributing mechanism of bacterial toxicity for some antimicrobial agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.