ArticleLab on a chip2010
Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Mesenchymal stem cells lineage and their role in disease development.Molecular medicine (Cambridge, Mass.) · 2024Review
- A bone-on-a-chip collagen hydrogel-based model using pre-differentiated adipose-derived stem cells for personalized bone tissue engineering.Journal of biomedical materials research. Part A · 2023Article
- "Chip-on-a-Transwell" Devices for User-Friendly Control of the Microenvironment of Cultured Cells.ACS applied bio materials · 2019Article
- Freeform micropatterning of living cells into cell culture medium using direct inkjet printing.Scientific reports · 2017Article
- In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- An open-chamber flow-focusing device for focal stimulation of micropatterned cells.Biomicrofluidics · 2016Article
- Generation and characterization of an immortalized human mesenchymal stromal cell line.Stem cells and development · 2014Article
- Regulation of Stem Cell Fate in a Three-Dimensional Micropatterned Dual-Crosslinked Hydrogel System.Advanced functional materials · 2013Article
- Microfluidic devices for cell cultivation and proliferation.Biomicrofluidics · 2013Review
- Epigenetic regulation and functional characterization of microRNA-142 in mesenchymal cells.PloS one · 2013Article
- Thick-tissue bioreactor as a platform for long-term organotypic culture and drug delivery.Lab on a chip · 2012Article
- Micro total analysis systems for cell biology and biochemical assays.Analytical chemistry · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Microtechnology offers great prospects for cellular research by enabling controlled experimental conditions that cannot be achieved by traditional methods. This study demonstrates the use of a microfluidic platform for long-term cultivation (3 weeks) of human mesenchymal stem-like cells (MSCs), a cell population of high interest for tissue engineering. The typical high motility of the MSCs required a strategy for preventing cells from inhabiting the feeding channels and thus interfere with a steady perfusion of medium to the cell cultivation chamber. Hence, a straightforward and long-term patterning method was developed and implemented for reliable cell positioning within the device. This method was based on the modification of a polystyrene substrate into cell supportive and non-supportive regions by the use of selective oxygen plasma treatment and the triblock copolymer Pluronic. Also, a novel and size-effective "flip-chip" set-up for operating the devices was invented. Successful and reproducible adipogenic and osteogenic differentiation of MSCs in the device was demonstrated, verifying that an adequate long-term microfluidic cultivation environment was obtained. Strengths of the experimental protocol include ease of fabrication and maintenance (gravity driven), good cell performance (viability/differentiation), as well as the possibility of exposing the culture to heterogeneous laminar flow for experimental purposes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.