ArticleInternational journal of molecular sciences2010
Electrostatic interactions of fluorescent molecules with dielectric interfaces studied by total internal reflection fluorescence correlation spectroscopy.
Article in International journal of molecular sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 20 citations in OpenAlex.
- Correlated diffusion in lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules.Scientific reports · 2019Article
- A nanofluidic device for parallel single nanoparticle catalysis in solution.Nature communications · 2019Article
- Simultaneous Surface-Near and Solution Fluorescence Correlation Spectroscopy.Journal of fluorescence · 2016Article
- Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents.Journal of molecular recognition : JMR · 2012Article
- Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy.Colloids and surfaces. B, Biointerfaces · 2011Article
- Measuring surface binding thermodynamics and kinetics by using total internal reflection with fluorescence correlation spectroscopy: practical considerations.The journal of physical chemistry. B · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electrostatic interactions between dielectric surfaces and different fluorophores used in ultrasensitive fluorescence microscopy are investigated using objective-based Total Internal Reflection Fluorescence Correlation Spectroscopy (TIR-FCS). The interfacial dynamics of cationic rhodamine 123 and rhodamine 6G, anionic/dianionic fluorescein, zwitterionic rhodamine 110 and neutral ATTO 488 are monitored at various ionic strengths at physiological pH. As analyzed by means of the amplitude and time-evolution of the autocorrelation function, the fluorescent molecules experience electrostatic attraction or repulsion at the glass surface depending on their charges. Influences of the electrostatic interactions are also monitored through the triplet-state population and triplet relaxation time, including the amount of detected fluorescence or the count-rate-per-molecule parameter. These TIR-FCS results provide an increased understanding of how fluorophores are influenced by the microenvironment of a glass surface, and show a promising approach for characterizing electrostatic interactions at interfaces.
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Registered trials
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