ArticleAntimicrobial agents and chemotherapy2010
Natamycin inhibits vacuole fusion at the priming phase via a specific interaction with ergosterol.
Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 101 citations in OpenAlex.
- New antifungal and less toxic zwitterionic derivatives of 26-membered polyene antibiotic - pimaricin containingJournal of enzyme inhibition and medicinal chemistry · 2026Article
- Antiviral Activity of Polyene Macrolides Against Newcastle Disease Virus: Computational and Experimental Insights.Molecules (Basel, Switzerland) · 2026Article
- Development and Characterization of Natamycin-Loaded Liposomes for Potential Topical Application: Influence of Preparation Method and Phospholipid Composition.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Fluorescent Probes Derived from the Polyene Class of Antifungal Drugs Reveal Distinct Localization Patterns and Resistance-Associated Vacuolar Sequestration in Candida Species.Angewandte Chemie (International ed. in English) · 2026Article
- Development and Validation of a QuEChERS-Based LC-MS/MS Method for Natamycin in Imported Agricultural Commodities in Korea.Foods (Basel, Switzerland) · 2025Article
- Phase separation of a master transcription factor targeted by a natural compound regulates fungal ergosterol homeostasis.Science China. Life sciences · 2025Article
- Engineered biosynthesis and characterization of disaccharide-pimaricin.Microbial cell factories · 2025Article
- Insights into the role of sterol metabolism in antifungal drug resistance: a mini-review.Frontiers in microbiology · 2024Review
- Late-stage diversification of bacterial natural products through biocatalysis.Frontiers in bioengineering and biotechnology · 2024Review
- CD3ε of a pan T cell marker involved in mouseInternational journal of ophthalmology · 2024Article
- Effect of Post-Polyketide Synthase Modification Groups on Property and Activity of Polyene Macrolides.Antibiotics (Basel, Switzerland) · 2023Review
- Article
- Polyenic Antibiotics and Other Antifungal Compounds Produced by HemolyticInternational journal of molecular sciences · 2022Review
- Amphotericin B resistance in Leishmania mexicana: Alterations to sterol metabolism and oxidative stress response.PLoS neglected tropical diseases · 2022Article
- Polyene Antibiotics Physical Chemistry and Their Effect on Lipid Membranes; Impacting Biological Processes and Medical Applications.Membranes · 2022Review
- Isolation, identification, biological characteristics, and antifungal efficacy of sodium bicarbonate combined with natamycin onFrontiers in microbiology · 2022Article
- Malignant Keratitis Caused by a Highly-Resistant Strain of Fusarium Tonkinense from theJournal of fungi (Basel, Switzerland) · 2021Article
- Natamycin: a natural preservative for food applications-a review.Food science and biotechnology · 2021Review
- Eye Infections Caused by Filamentous Fungi: Spectrum and Antifungal Susceptibility of the Prevailing Agents in Germany.Journal of fungi (Basel, Switzerland) · 2021Article
- Sterol Sponge Mechanism Is Conserved for Glycosylated Polyene Macrolides.ACS central science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antifungal antibiotic natamycin belongs to the family of polyene antibiotics. Its antifungal activity arises via a specific interaction with ergosterol in the plasma membrane (te Welscher et al., J. Biol. Chem. 283:6393-6401, 2008). However, this activity does not involve disruption of the membrane barrier function, a well-known property of other members of the polyene antibiotic family, such as filipin and nystatin. Here we tested the effect of natamycin on vacuole membrane fusion, which is known to be ergosterol dependent. Natamycin blocked the fusion of isolated vacuoles without compromising the barrier function of the vacuolar membrane. Sublethal doses of natamycin perturbed the cellular vacuole morphology, causing the formation of many more small vacuolar structures in yeast cells. Using vacuoles isolated from yeast strains deficient in the ergosterol biosynthesis pathway, we showed that the inhibitory activity of natamycin was dependent on the presence of specific chemical features in the structure of ergosterol that allow the binding of natamycin. We found that natamycin inhibited the priming stage of vacuole fusion. Similar results were obtained with nystatin. These results suggest a novel mode of action of natamycin and perhaps all polyene antibiotics, which involves the impairment of membrane fusion via perturbation of ergosterol-dependent priming reactions that precede membrane fusion, and they may point to an effect of natamycin on ergosterol-dependent protein function in general.
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