ArticleProceedings of the National Academy of Sciences of the United States of America2010
Steric confinement of proteins on lipid membranes can drive curvature and tubulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
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- Solvent-Mediated Dewetting Principles for Cell-Sized Liposome Formation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions.Small methods · 2025Article
- Analysis of native Ist1 dynamics reveals multiple pools of ESCRT-III on endosomes.The Journal of cell biology · 2025Article
- Cellular Signaling at the Nano-Bio Interface: Spotlighting Membrane Curvature.Annual review of physical chemistry · 2025Review
- Intrinsically disordered region amplifies membrane remodeling to augment selective ER-phagy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Triacylglycerol-droplet-induced bilayer spontaneous curvature in giant unilamellar vesicles.Biophysical journal · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Protein-membrane interactions: sensing and generating curvature.Trends in biochemical sciences · 2024Review
- Interplay of α-synuclein with Lipid Membranes: Cooperative Adsorption, Membrane Remodeling and Coaggregation.JACS Au · 2024Review
- Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation.International journal of molecular sciences · 2023Article
- Dynamics of upstream ESCRT organization at the HIV-1 budding site.Biophysical journal · 2023Article
- The ins and outs of membrane bending by intrinsically disordered proteins.Science advances · 2023Article
- Formation of protein-mediated bilayer tubes is governed by a snapthrough transition.Soft matter · 2023Article
- Coupling of protein condensates to ordered lipid domains determines functional membrane organization.Science advances · 2023Article
- Propranolol induces large-scale remodeling of lipid bilayers: tubules, patches, and holes.RSC advances · 2023Article
- Regulation of membrane protein structure and function by their lipid nano-environment.Nature reviews. Molecular cell biology · 2023Review
41 more citing papers are in PubMed but not listed here.
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3 authors.
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Abstract
Deformation of lipid membranes into curved structures such as buds and tubules is essential to many cellular structures including endocytic pits and filopodia. Binding of specific proteins to lipid membranes has been shown to promote membrane bending during endocytosis and transport vesicle formation. Additionally, specific lipid species are found to colocalize with many curved membrane structures, inspiring ongoing exploration of a variety of roles for lipid domains in membrane bending. However, the specific mechanisms by which lipids and proteins collaborate to induce curvature remain unknown. Here we demonstrate a new mechanism for induction and amplification of lipid membrane curvature that relies on steric confinement of protein binding on membrane surfaces. Using giant lipid vesicles that contain domains with high affinity for his-tagged proteins, we show that protein crowding on lipid domain surfaces creates a protein layer that buckles outward, spontaneously bending the domain into stable buds and tubules. In contrast to previously described bending mechanisms relying on local steric interactions between proteins and lipids (i.e. helix insertion into membranes), this mechanism produces tubules whose dimensions are defined by global parameters: domain size and membrane tension. Our results suggest the intriguing possibility that confining structures, such as lipid domains and protein lattices, can amplify membrane bending by concentrating the steric interactions between bound proteins. This observation highlights a fundamental physical mechanism for initiation and control of membrane bending that may help explain how lipids and proteins collaborate to create the highly curved structures observed in vivo.
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