ArticleProceedings of the National Academy of Sciences of the United States of America2010
Why Hofmeister effects of many salts favor protein folding but not DNA helix formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Fractional Ion Contributions to Mixed Electrolyte Ion Atmospheres from Experimental Thermodynamic Data.The journal of physical chemistry. B · 2026Article
- Ion Adsorption at Surfaces from Local and Global Electroneutrality Constraints.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry.ChemistryOpen · 2025Article
- Direct comparison of the structural dynamics between spontaneous and ligand-induced folding of staphylococcal nuclease.Protein science : a publication of the Protein Society · 2025Article
- Ion-Ion Association in Bulk Mixed Electrolytes Using Global and Local Electroneutrality Constraints.The journal of physical chemistry. B · 2025Article
- The Streptococcus phage protein paratox is an intrinsically disordered protein.Protein science : a publication of the Protein Society · 2024Article
- Anion Binding to Ammonium and Guanidinium Hosts: Implications for the Reverse Hofmeister Effects Induced by Lysine and Arginine Residues.The Journal of organic chemistry · 2024Article
- Dual Effect of Secondary Solutes on Binding Equilibria: Contributions from Solute-Reactant Interactions and Solute-Water Interactions.ACS omega · 2024Article
- Salt Effects on Caffeine across Concentration Regimes.The journal of physical chemistry. B · 2023Article
- Structure and Formation of Z-DNA and Z-RNA.Molecules (Basel, Switzerland) · 2023Review
- Correlation coefficient-directed label-free characterization of native proteins by surface-enhanced Raman spectroscopy.Chemical science · 2022Article
- The Metabolome Weakens RNA Thermodynamic Stability and Strengthens RNA Chemical Stability.Biochemistry · 2022Article
- DNA G-quadruplexes are uniquely stable in the presence of denaturants and monovalent cations.Biochemistry and biophysics reports · 2022Article
- Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.Chembiochem : a European journal of chemical biology · 2022Article
- Denaturation of proteins: electrostatic effectsRSC advances · 2022Article
- Systematic Parameterization of Ion-Surfactant Interactions in Dissipative Particle Dynamics Using Setschenow Coefficients.The journal of physical chemistry. B · 2022Article
- Hydrogen Bonding and Solvation of a Proline-Based Peptide Model in Salt Solutions.Life (Basel, Switzerland) · 2021Article
- Reentrant liquid condensate phase of proteins is stabilized by hydrophobic and non-ionic interactions.Nature communications · 2021Article
- Unnatural bases for recognition of noncoding nucleic acid interfaces.Biopolymers · 2021Review
- Revealing the Hidden Sensitivity of Intrinsically Disordered Proteins to their Chemical Environment.The journal of physical chemistry letters · 2020Article
5 more citing papers are in PubMed but not listed here.
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7 authors.
Funding
Abstract
The majority ( approximately 70%) of surface buried in protein folding is hydrocarbon, whereas in DNA helix formation, the majority ( approximately 65%) of surface buried is relatively polar nitrogen and oxygen. Our previous quantification of salt exclusion from hydrocarbon (C) accessible surface area (ASA) and accumulation at amide nitrogen (N) and oxygen (O) ASA leads to a prediction of very different Hofmeister effects on processes that bury mostly polar (N, O) surface compared to the range of effects commonly observed for processes that bury mainly nonpolar (C) surface, e.g., micelle formation and protein folding. Here we quantify the effects of salts on folding of the monomeric DNA binding domain (DBD) of lac repressor (lac DBD) and on formation of an oligomeric DNA duplex. In accord with this prediction, no salt investigated has a stabilizing Hofmeister effect on DNA helix formation. Our ASA-based analyses of model compound data and estimates of the surface area buried in protein folding and DNA helix formation allow us to predict Hofmeister effects on these processes. We observe semiquantitative to quantitative agreement between these predictions and the experimental values, obtained from a novel separation of coulombic and Hofmeister effects. Possible explanations of deviations, including salt-dependent unfolded ensembles and interactions with other types of surface, are discussed.
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