ArticleProceedings of the National Academy of Sciences of the United States of America2010
Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 271 papers.
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Who cites it
271 citing papers in PubMed, 571 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- CDPK gene family in Camellia nitidissima: genome-wide identification and expression analysis during petal development and hormone treatments.BMC plant biology · 2026Article
- SlSLAH2 mediates malate exudation and contributes to aluminum tolerance.Nature communications · 2026Article
- A guard cell carbonic anhydrase binds and regulates SLAC1 separate from its catalytic activity.Nature communications · 2026Article
- Genome-wide identification of theFrontiers in plant science · 2026Article
- The transcriptome-identified gene HaSAPK3.8 of sunflower (Helianthus annuus L.) confers drought resistance in tobacco.BMC plant biology · 2025Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- Structural insights into the coordinated regulation of the SLAH family in Arabidopsis thaliana.Nature communications · 2025Article
- Article
- Zymoseptoria tritici stealth infection is facilitated by stage-specific downregulation of a β-glucanase.The New phytologist · 2025Article
- OPEN STOMATA 1 activates SLAC1 anion channel primarily through CPK15 in ABA-induced stomatal closure inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2025Article
- CaNature communications · 2025Article
- SAL1-PAP retrograde signaling orchestrates photosynthetic and extracellular reactive oxygen species for stress responses.The Plant journal : for cell and molecular biology · 2025Article
- A SnRK2-HAK regulatory module confers natural variation of salt tolerance in maize.Nature communications · 2025Article
- ZmGCT1/2 negatively regulate drought tolerance in maize by inhibiting ZmSLAC1 to maintain guard cell turgor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- SnRK2s: Kinases or Substrates?Plants (Basel, Switzerland) · 2025Review
- Wheat TaPYL9-involved signalling pathway impacts plant drought response through regulating distinct osmotic stress-associated physiological indices.Plant biotechnology journal · 2025Article
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
- Potassium extrusion by plant cells: evolution from an emergency valve to a driver of long-distance transport.The New phytologist · 2025Review
211 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In response to drought stress, the phytohormone abscisic acid (ABA) induces stomatal closure. Thereby the stress hormone activates guard cell anion channels in a calcium-dependent, as well as -independent, manner. Open stomata 1 protein kinase (OST1) and ABI1 protein phosphatase (ABA insensitive 1) represent key components of calcium-independent ABA signaling. Recently, the guard cell anion channel SLAC1 was identified. When expressed heterologously SLAC1 remained electrically silent. Upon coexpression with Ca(2+)-independent OST1, however, SLAC1 anion channels appear activated in an ABI1-dependent manner. Mutants lacking distinct calcium-dependent protein kinases (CPKs) appeared impaired in ABA stimulation of guard cell ion channels, too. To study SLAC1 activation via the calcium-dependent ABA pathway, we studied the SLAC1 response to CPKs in the Xenopus laevis oocyte system. Split YFP-based protein-protein interaction assays, using SLAC1 as the bait, identified guard cell expressed CPK21 and 23 as major interacting partners. Upon coexpression of SLAC1 with CPK21 and 23, anion currents document SLAC1 stimulation by these guard cell protein kinases. Ca(2+)-sensitive activation of SLAC1, however, could be assigned to the CPK21 pathway only because CPK23 turned out to be rather Ca(2+)-insensitive. In line with activation by OST1, CPK activation of the guard cell anion channel was suppressed by ABI1. Thus the CPK and OST1 branch of ABA signal transduction in guard cells seem to converge on the level of SLAC1 under the control of the ABI1/ABA-receptor complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.