ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2010
Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Canine parvovirus infection affects host cell nucleolar organization and ribosome biogenesis.Communications biology · 2026Article
- Aged gastrocnemius muscle of mice positively responds to a late onset adapted physical training.Frontiers in cell and developmental biology · 2023Article
- Selective Targeting of Cancerous Mitochondria and Suppression of Tumor Growth Using Redox-Active Treatment Adjuvant.Oxidative medicine and cellular longevity · 2020Article
- IFN-γ restores the impaired function of RNase L and induces mitochondria-mediated apoptosis in lung cancer.Cell death & disease · 2019Article
- Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia.Cellular and molecular life sciences : CMLS · 2014Article
- The nucleolus of Caenorhabditis elegans.Journal of biomedicine & biotechnology · 2012Review
- The bladder tumor suppressor protein TERE1 (UBIAD1) modulates cell cholesterol: implications for tumor progression.DNA and cell biology · 2011Article
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Authors and funding
8 authors.
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Abstract
Ascorbate and menadione (Apatone) in a ratio of 100:1 kills tumor cells by autoschizis. In this study, vitamin-induced changes in nucleolar structure were evaluated as markers of autoschizis. Human bladder carcinoma (T24) cells were overlain with vitamins or with culture medium. Supernatants were removed at 1-hr intervals from 1 to 4 hr, and the cells were washed with PBS and prepared for assay. Apatone produced marked alterations in nucleolar structure including redistribution of nucleolar components, formation of ring-shaped nucleoli, condensation and increase of the proportion of perinucleolar chromatin, and the enlargement of nucleolar fibrillar centers. Immunogold labeling of the nucleolar rRNA revealed a granular localization in treated and sham-treated cells, and immunogold labeling of the rDNA revealed a shift from the fibrillar centers to the condensed perinucleolar chromatin. Fibrillarin staining shifted from the fibrillar centers and adjacent regions to a more homogeneous staining of the entire nucleolus and was consistent with the percentage of autoschizic cells detected by flow cytometry. Because autoschizis entails sequential reactivation of DNase I and DNase II, and because the fibrillarin redistribution following DNase I and Apatone treatment is identical, it appears that the nucleolar and fibrillarin changes are markers of autoschizis.
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