ArticleNucleic acids research2010
Functional selection and systematic analysis of intronic splicing elements identify active sequence motifs and associated splicing factors.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- RNA-Based Therapies for Inherited Metabolic Disorders.Journal of inherited metabolic disease · 2026Review
- Massively parallel assay of human splice variants reveals cis-regulatory drivers of disease-associated and cell type-specific splicing regulation.bioRxiv : the preprint server for biology · 2025Article
- Decoding biology with massively parallel reporter assays and machine learning.Genes & development · 2024Review
- Mechanism and modeling of human disease-associated near-exon intronic variants that perturb RNA splicing.Nature structural & molecular biology · 2022Article
- Systematic characterization of short intronic splicing-regulatory elements in SMN2 pre-mRNA.Nucleic acids research · 2022Article
- Vex-seq: high-throughput identification of the impact of genetic variation on pre-mRNA splicing efficiency.Genome biology · 2018Article
- Saturation mutagenesis reveals manifold determinants of exon definition.Genome research · 2018Article
- Mammalian synthetic biology for studying the cell.The Journal of cell biology · 2017Review
- Splicing defects caused by exonic mutations in PKD1 as a new mechanism of pathogenesis in autosomal dominant polycystic kidney disease.RNA biology · 2015Article
- Pre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate.Genes & development · 2014Article
- Optimal antisense target reducing INS intron 1 retention is adjacent to a parallel G quadruplex.Nucleic acids research · 2014Article
- Systematical identification of splicing regulatory cis-elements and cognate trans-factors.Methods (San Diego, Calif.) · 2014Article
- Yin Yang 1 intronic binding sequences and splicing elicit intron-mediated enhancement of ubiquitin C gene expression.PloS one · 2013Article
- A complex network of factors with overlapping affinities represses splicing through intronic elements.Nature structural & molecular biology · 2013Article
- An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution.Science (New York, N.Y.) · 2012Article
- Intronic splicing enhancers, cognate splicing factors and context-dependent regulation rules.Nature structural & molecular biology · 2012Article
- The function of introns.Frontiers in genetics · 2012Article
- Rational design of a small molecule-responsive intramer controlling transgene expression in mammalian cells.Nucleic acids research · 2011Article
- Reprogramming cellular behavior with RNA controllers responsive to endogenous proteins.Science (New York, N.Y.) · 2010Article
- Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the critical role of pre-mRNA splicing in generating proteomic diversity and regulating gene expression, the sequence composition and function of intronic splicing regulatory elements (ISREs) have not been well elucidated. Here, we employed a high-throughput in vivo Screening PLatform for Intronic Control Elements (SPLICE) to identify 125 unique ISRE sequences from a random nucleotide library in human cells. Bioinformatic analyses reveal consensus motifs that resemble splicing regulatory elements and binding sites for characterized splicing factors and that are enriched in the introns of naturally occurring spliced genes, supporting their biological relevance. In vivo characterization, including an RNAi silencing study, demonstrate that ISRE sequences can exhibit combinatorial regulatory activity and that multiple trans-acting factors are involved in the regulatory effect of a single ISRE. Our work provides an initial examination into the sequence characteristics and function of ISREs, providing an important contribution to the splicing code.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.