ArticleNucleic acids research2010
A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 29 papers.
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29 citing papers in PubMed, 53 citations in OpenAlex.
- Dnmt3a2 expression during embryonic development is required for phenotypic stability.Communications biology · 2025Article
- Dynamic allelic expression in mouse mammary glands across the adult developmental cycle.Nucleic acids research · 2025Article
- Article
- Imprinted Long Non-Coding RNAs in Mammalian Development and Disease.International journal of molecular sciences · 2023Review
- The DNMT3A ADD domain is required for efficient de novo DNA methylation and maternal imprinting in mouse oocytes.PLoS genetics · 2023Article
- MC profiling: a novel approach to analyze DNA methylation heterogeneity in genome-wide bisulfite sequencing data.NAR genomics and bioinformatics · 2022Article
- Identification of the novel Ido1 imprinted locus and its potential epigenetic role in pregnancy loss.Human molecular genetics · 2019Article
- Cell proliferation potency is independent of FGF4 signaling in trophoblast stem cells derived from androgenetic embryos.The Journal of reproduction and development · 2016Article
- Glycogen synthase kinase-3 (Gsk-3) plays a fundamental role in maintaining DNA methylation at imprinted loci in mouse embryonic stem cells.Molecular biology of the cell · 2015Article
- Allele-specific binding of ZFP57 in the epigenetic regulation of imprinted and non-imprinted monoallelic expression.Genome biology · 2015Article
- Genes Downregulated in Endometriosis Are Located Near the Known Imprinting Genes.Reproductive sciences (Thousand Oaks, Calif.) · 2014Article
- Article
- Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of human imprinting and suggests a germline methylation-independent mechanism of establishment.Genome research · 2014Article
- The Gpr1/Zdbf2 locus provides new paradigms for transient and dynamic genomic imprinting in mammals.Genes & development · 2014Article
- Characterization of the imprinting signature of mouse embryo fibroblasts by RNA deep sequencing.Nucleic acids research · 2014Article
- Association of childhood chronic physical aggression with a DNA methylation signature in adult human T cells.PloS one · 2014Article
- De novo DNA methylation in the male germ line occurs by default but is excluded at sites of H3K4 methylation.Cell reports · 2013Article
- Epigenetic and transcriptional features of the novel human imprinted lncRNA GPR1AS suggest it is a functional ortholog to mouse Zdbf2linc.Epigenetics · 2013Article
- Stability of genomic imprinting in human induced pluripotent stem cells.BMC genetics · 2013Article
- Identification of novel imprinted differentially methylated regions by global analysis of human-parthenogenetic-induced pluripotent stem cells.Stem cell reports · 2013Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. To date, four paternally methylated DMRs have been identified in screens based on conventional approaches. These DMRs are linked to the imprinted genes H19, Gtl2 (IG-DMR), Rasgrf1 and, most recently, Zdbf2 which encodes zinc finger, DBF-type containing 2. In this study, we applied a novel methylated-DNA immunoprecipitation-on-chip (meDIP-on-chip) method to genomic DNA from mouse parthenogenetic- and androgenetic-derived stem cells and sperm and identified 458 putative DMRs. This included the majority of known DMRs. We further characterized the paternally methylated Zdbf2/ZDBF2 DMR. In mice, this extensive germ line DMR spanned 16 kb and possessed an unusual tripartite structure. Methylation was dependent on DNA methyltransferase 3a (Dnmt3a), similar to H19 DMR and IG-DMR. In both humans and mice, the adjacent gene, Gpr1/GPR1, which encodes a G-protein-coupled receptor 1 protein with transmembrane domain, was also imprinted and paternally expressed. The Gpr1-Zdbf2 domain was most similar to the Rasgrf1 domain as both DNA methylation and the actively expressed allele were in cis on the paternal chromosome. This work demonstrates the effectiveness of meDIP-on-chip as a technique for identifying DMRs.
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