ReviewNucleic acids research2010
Strategies for protein synthetic biology.
Review in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Structural ontogeny of protein-protein interactions.Science (New York, N.Y.) · 2026Article
- Multi-Omic Analysis of the Differences in Growth and Metabolic Mechanisms Between Chinese Domestic Cattle and Simmental Crossbred Cattle.International journal of molecular sciences · 2025Article
- Differential amino acid usage leads to ubiquitous edge effect in proteomes across domains of life that can be explained by amino acid secondary structure propensities.Scientific reports · 2024Article
- A Ubiquitin-Based Module Directing Protein-Protein Interactions in Chloroplasts.International journal of molecular sciences · 2023Article
- Designing and computational analyzing of chimeric long-lasting GLP-1 receptor agonists for type 2 diabetes.Scientific reports · 2023Article
- Synthetic Protein Circuits and Devices Based on Reversible Protein-Protein Interactions: An Overview.Life (Basel, Switzerland) · 2021Review
- An engineered protein-phosphorylation toggle network with implications for endogenous network discovery.Science (New York, N.Y.) · 2021Article
- Protein assembly systems in natural and synthetic biology.BMC biology · 2020Review
- iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains.Nucleic acids research · 2020Article
- Unexpected Roles of a Tether Harboring a Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis.ACS catalysis · 2019Article
- Engineering protein-protein devices for multilayered regulation of mRNA translation using orthogonal proteases in mammalian cells.Nature communications · 2018Article
- Dynamic protein assembly by programmable DNA strand displacement.Nature chemistry · 2018Article
- Programmed Self-Assembly of a Biochemical and Magnetic Scaffold to Trigger and Manipulate Microtubule Structures.Scientific reports · 2017Article
- Designer cell signal processing circuits for biotechnology.New biotechnology · 2015Review
- Combining random gene fission and rational gene fusion to discover near-infrared fluorescent protein fragments that report on protein-protein interactions.ACS synthetic biology · 2015Article
- SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution.Protein engineering, design & selection : PEDS · 2014Article
- Article
- Challenges and opportunities in synthetic biology for chemical engineers.Chemical engineering science · 2013Article
- Tuning response curves for synthetic biology.ACS synthetic biology · 2013Article
- Rewiring cell signalling through chimaeric regulatory protein engineering.Biochemical Society transactions · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins are the most versatile among the various biological building blocks and a mature field of protein engineering has lead to many industrial and biomedical applications. But the strength of proteins-their versatility, dynamics and interactions-also complicates and hinders systems engineering. Therefore, the design of more sophisticated, multi-component protein systems appears to lag behind, in particular, when compared to the engineering of gene regulatory networks. Yet, synthetic biologists have started to tinker with the information flow through natural signaling networks or integrated protein switches. A successful strategy common to most of these experiments is their focus on modular interactions between protein domains or domains and peptide motifs. Such modular interaction swapping has rewired signaling in yeast, put mammalian cell morphology under the control of light, or increased the flux through a synthetic metabolic pathway. Based on this experience, we outline an engineering framework for the connection of reusable protein interaction devices into self-sufficient circuits. Such a framework should help to 'refacture' protein complexity into well-defined exchangeable devices for predictive engineering. We review the foundations and initial success stories of protein synthetic biology and discuss the challenges and promises on the way from protein- to protein systems design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.