ArticleBMC genomics2010
A toolkit for rapid gene mapping in the nematode Caenorhabditis briggsae.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- A gap-free, telomere-to-telomere genome assembly for the Caenorhabditis briggsae reference strain AF16.G3 (Bethesda, Md.) · 2026Article
- A gap-free, telomere-to-telomere genome assembly for thebioRxiv : the preprint server for biology · 2026Article
- Heat tolerance and genetic adaptations in Caenorhabditis briggsae: insights from comparative studies with Caenorhabditis elegans.Genetics · 2025Article
- Natural variation in infection specificity of Caenorhabditis briggsae isolates by two RNA viruses.PLoS pathogens · 2024Article
- Reciprocal restriction fragment length polymorphism (RFLP) analysis reveals mitochondrial heteroplasmy inmicroPublication biology · 2024Article
- Characterization of two newmicroPublication biology · 2023Article
- Genetic architecture and temporal analysis of Caenorhabditis briggsae hybrid developmental delay.PloS one · 2022Article
- Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans.Scientific reports · 2021Article
- Article
- Temperature-dependent mitochondrial-nuclear epistasis.microPublication biology · 2019Article
- Genetically Distinct Behavioral Modules Underlie Natural Variation in Thermal Performance Curves.G3 (Bethesda, Md.) · 2019Article
- The Genetic Architecture of Intra-Species Hybrid Mito-Nuclear Epistasis.Frontiers in genetics · 2018Article
- Article
- Differing roles for sur-2/MED23 in C. elegans and C. briggsae vulval development.Development genes and evolution · 2017Article
- Revisiting Suppression of Interspecies Hybrid Male Lethality inG3 (Bethesda, Md.) · 2017Article
- Paternal Mitochondrial Transmission in Intra-Species Caenorhabditis briggsae Hybrids.Molecular biology and evolution · 2016Article
- Mitochondrial-Nuclear Epistasis Impacts Fitness and Mitochondrial Physiology of Interpopulation Caenorhabditis briggsae Hybrids.G3 (Bethesda, Md.) · 2015Article
- Full-genome evolutionary histories of selfing, splitting, and selection in Caenorhabditis.Genome research · 2015Article
- A Genome-wide hybrid incompatibility landscape between Caenorhabditis briggsae and C. nigoni.PLoS genetics · 2015Article
- Comparative functional characterization of the CSR-1 22G-RNA pathway in Caenorhabditis nematodes.Nucleic acids research · 2015Article
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe nematode C. briggsae serves as a useful model organism for comparative analysis of developmental and behavioral processes. The amenability of C. briggsae to genetic manipulations and the availability of its genome sequence have prompted researchers to study evolutionary changes in gene function and signaling pathways. These studies rely on the availability of forward genetic tools such as mutants and mapping markers.
resultsWe have computationally identified more than 30,000 polymorphisms (SNPs and indels) in C. briggsae strains AF16 and HK104. These include 1,363 SNPs that change restriction enzyme recognition sites (snip-SNPs) and 638 indels that range between 7 bp and 2 kb. We established bulk segregant and single animal-based PCR assay conditions and used these to test 107 polymorphisms. A total of 75 polymorphisms, consisting of 14 snip-SNPs and 61 indels, were experimentally confirmed with an overall success rate of 83%. The utility of polymorphisms in genetic studies was demonstrated by successful mapping of 12 mutations, including 5 that were localized to sub-chromosomal regions. Our mapping experiments have also revealed one case of a misassembled contig on chromosome 3.
conclusionsWe report a comprehensive set of polymorphisms in C. briggsae wild-type strains and demonstrate their use in mapping mutations. We also show that molecular markers can be useful tools to improve the C. briggsae genome sequence assembly. Our polymorphism resource promises to accelerate genetic and functional studies of C. briggsae genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.