ArticleACS nano2010
Enhancement of in vitro translation by gold nanoparticle--DNA conjugates.
Article in ACS nano, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 60 citations in OpenAlex.
- Uptake and stability of DNA nanostructures in cells: a cross-sectional overview of the current state of the art.Nanoscale · 2023Review
- Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs.Nature communications · 2021Article
- Biomedical applications of mRNA nanomedicine.Nano research · 2018Article
- A comparison of nanoparticle-antibody conjugation strategies in sandwich immunoassays.Journal of immunoassay & immunochemistry · 2017Article
- Inhibition of Xenograft tumor growth by gold nanoparticle-DNA oligonucleotide conjugates-assisted delivery of BAX mRNA.PloS one · 2013Article
- Protective effect of the apoptosis-sensing nanoparticle AnxCLIO-Cy5.5.Nanomedicine : nanotechnology, biology, and medicine · 2012Article
- Review
- Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids.PloS one · 2012Article
- Oligonucleotide delivery by cell-penetrating "striped" nanoparticles.Angewandte Chemie (International ed. in English) · 2011Article
- Nanoscale interfaces to biology.Current opinion in chemical biology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Gold nanoparticle (AuNP)-DNA conjugates can enhance in vitro translation of a protein. Enhancement occurs via a combination of nonspecific adsorption of translation-related molecules and the ribosome to the AuNP-DNA and specific binding to the mRNA of interest. AuNP-DNA conjugates enhanced protein production of fluorescent proteins (mCherry, eGFP) in retic lysate mixes by 65-100%. Gel electrophoresis was used to probe nonspecific adsorption of the AuNP-DNA conjugates to the translation machinery. It was determined that nonspecific adsorption is critical for enhancement, and if it was eliminated, expression enhancement did not occur. The interaction of the mRNA with the DNA on the AuNP surface influenced the amount of enhancement and was probed by expression in the presence of RNase H. These results suggest that higher translation enhancement occurs when the DNA on the AuNP forms an incomplete duplex with the mRNA. Tuning the balance between nonspecific adsorption and specific binding of the AuNP-DNA conjugates could result in the translation enhancement of a specific gene in a mixture.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.