ReviewActa crystallographica. Section D, Biological crystallography2010
Selenium incorporation using recombinant techniques.
Review in Acta crystallographica. Section D, Biological crystallography, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa.Nucleic acids research · 2024Article
- Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit inbioRxiv : the preprint server for biology · 2024Article
- Decrypting the programming of β-methylation in virginiamycin M biosynthesis.Nature communications · 2023Article
- Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.Scientific reports · 2021Article
- Structural basis of the membrane intramolecular transacylase reaction responsible for lyso-form lipoprotein synthesis.Nature communications · 2021Article
- How to limit the speed of a motor: the intricate regulation of the XPB ATPase and translocase in TFIIH.Nucleic acids research · 2020Article
- Structural Characterization of the N-Terminal Domain of theACS omega · 2020Article
- Structural insights into phosphopantetheinyl hydrolase PptH from Mycobacterium tuberculosis.Protein science : a publication of the Protein Society · 2020Article
- Structural and Functional Analyses of Human ChaC2 in Glutathione Metabolism.Biomolecules · 2019Article
- Crystal structure of the transcriptional repressor DdrO: insight into the metalloprotease/repressor-controlled radiation response in Deinococcus.Nucleic acids research · 2019Article
- Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS.Acta crystallographica. Section D, Structural biology · 2019Article
- Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.Journal of applied crystallography · 2019Article
- Highly Efficient Preparation of Cyclic DinucleotidesFrontiers in microbiology · 2019Article
- Post-Translational Backbone Engineering through Selenomethionine-Mediated Incorporation of Freidinger Lactams.Angewandte Chemie (International ed. in English) · 2018Article
- Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.Journal of visualized experiments : JoVE · 2018Article
- Structure-function analyses of a PL24 family ulvan lyase reveal key features and suggest its catalytic mechanism.The Journal of biological chemistry · 2018Article
- The molecular structure of the glycoside hydrolase domain of Cwp19 from Clostridium difficile.The FEBS journal · 2017Article
- Structural insights into the committed step of bacterial phospholipid biosynthesis.Nature communications · 2017Article
- CIDE domains form functionally important higher-order assemblies for DNA fragmentation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using selenomethionine to phase macromolecular structures is common practice in structure determination, along with the use of selenocysteine. Selenium is consequently the most commonly used heavy atom for MAD. In addition to the well established recombinant techniques for the incorporation of selenium in prokaryal expression systems, there have been recent advances in selenium labelling in eukaryal expression, which will be discussed. Tips and things to consider for the purification and crystallization of seleno-labelled proteins are also included.
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