ArticleMolecular endocrinology (Baltimore, Md.)2010
Generation of ES cells for conditional expression of nuclear receptors and coregulators in vivo.
Article in Molecular endocrinology (Baltimore, Md.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 35 citations in OpenAlex.
- Identification of Key Differentially Expressed Genes During Early Sex Determination in Chicken Embryos.International journal of molecular sciences · 2025Article
- Muscle metabolic resilience and enhanced exercise adaptation by Esr1-induced remodeling of mitochondrial cristae-nucleoid architecture in males.Cell reports. Medicine · 2025Article
- NR2F1 shapes mitochondria in the mouse brain, providing new insights into Bosch-Boonstra-Schaaf optic atrophy syndrome.Disease models & mechanisms · 2023Article
- The NR2F2-HAND2 signaling axis regulates progesterone actions in the uterus at early pregnancy.Frontiers in endocrinology · 2023Article
- Conditional ERK3 overexpression cooperates with PTEN deletion to promote lung adenocarcinoma formation in mice.Molecular oncology · 2022Article
- Article
- Constitutive expression of Steroidogenic factor-1 (NR5A1) disrupts ovarian functions, fertility, and metabolic homeostasis in female mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- COUP-TFI specifies the medial entorhinal cortex identity and induces differential cell adhesion to determine the integrity of its boundary with neocortex.Science advances · 2021Article
- Opposing Functions of BRD4 Isoforms in Breast Cancer.Molecular cell · 2020Article
- Dysregulation of hypothalamic-pituitary estrogen receptor α-mediated signaling causes episodic LH secretion and cystic ovary.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- A mouse model engineered to conditionally express the progesterone receptor-B isoform.Genesis (New York, N.Y. : 2000) · 2018Article
- Generation of Mouse for Conditional Expression of Forkhead Box A2.Endocrinology · 2018Article
- Dysregulation of nuclear receptor COUP-TFII impairs skeletal muscle development.Scientific reports · 2017Article
- Decreased epithelial progesterone receptor A at the window of receptivity is required for preparation of the endometrium for embryo attachment.Biology of reproduction · 2017Article
- COUP-TFII regulates satellite cell function and muscular dystrophy.The Journal of clinical investigation · 2016Article
- Endometrial Expression of Steroidogenic Factor 1 Promotes Cystic Glandular Morphogenesis.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Choose your destiny: Make a cell fate decision with COUP-TFII.The Journal of steroid biochemistry and molecular biology · 2016Review
- Estrogen receptor-α in medial amygdala neurons regulates body weight.The Journal of clinical investigation · 2015Article
- A microRNA-Hippo pathway that promotes cardiomyocyte proliferation and cardiac regeneration in mice.Science translational medicine · 2015Article
- Mig-6 suppresses endometrial cancer associated with Pten deficiency and ERK activation.Cancer research · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Nuclear receptors and coregulators orchestrate diverse aspects of biological functions and inappropriate expression of these factors often associates with human diseases. The present study describes a conditional overexpression system consisting of a minigene located at the Rosa26 locus in the genome of mouse embryonic stem (ES) cells. Before activation, the minigene is silent due to a floxed STOP cassette inserted between the promoter and the transgene. Upon cre-mediated excision of the STOP cassette, the minigene constitutively expresses the tagged transgene driven by the ubiquitous CAGGS promoter. Thus, this system can be used to express target gene in any tissue in a spatial and/or temporal manner if respective cre mouse lines are available. Serving as proof of principle, the CAG-S-hCOUP-TFI allele was generated in ES cells and subsequently in mice. This allele was capable of conditionally overexpressing human chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI) in all tissues tested upon activation by cre drivers. This allele was further subjected to address functionality of expressed COUP-TFI and the functional similarity between COUP-TFI and COUP-TFII. Expression of COUP-TFI in COUP-TFII-ablated uterus suppressed aberrant estrogen receptor-alpha activities and rescued implantation and decidualization defects of COUP-TFII mutants, suggesting that COUP-TFI and COUP-TFII are able to functionally compensate for each other in the uterus. A toolbox currently under construction will contain ES cell lines for overexpressing all 48 nuclear receptors and selected 10 coregulators. Upon completion, it will be a very valuable resource for the scientific community. Several ES cells are currently available for distribution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.