ArticleApplied and environmental microbiology2010
Study of matrix additives for sensitive analysis of lipid A by matrix-assisted laser desorption ionization mass spectrometry.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Altering Lipid A Precursor Ion Types in the Gas Phase for In-Depth Structural Elucidation via Tandem Mass Spectrometry.Analytical chemistry · 2025Article
- Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive.Analytical chemistry · 2023Article
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- A Klebsiella pneumoniae DedA family membrane protein is required for colistin resistance and for virulence in wax moth larvae.Scientific reports · 2021Article
- Current Scenario and Challenges in the Direct Identification of Microorganisms Using MALDI TOF MS.Microorganisms · 2021Review
- Article
- Analyses of Lipid A Diversity in Gram-Negative Intestinal Bacteria Using Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.Metabolites · 2021Article
- Colistin Dependence in Extensively Drug-ResistantInternational journal of molecular sciences · 2021Article
- A concise review on lipidomics analysis in biological samples.ADMET & DMPK · 2021Review
- The Influence of Different Forms of Silver on Selected Pathogenic Bacteria.Materials (Basel, Switzerland) · 2020Article
- HupA, the main undecaprenyl pyrophosphate and phosphatidylglycerol phosphate phosphatase in Helicobacter pylori is essential for colonization of the stomach.PLoS pathogens · 2019Article
- A DedA Family Membrane Protein Is Required forFrontiers in microbiology · 2019Article
- Mass spectrometry-based shotgun lipidomics - a critical review from the technical point of view.Analytical and bioanalytical chemistry · 2018Review
- Article
- Classification of Bacillus and Brevibacillus species using rapid analysis of lipids by mass spectrometry.Analytical and bioanalytical chemistry · 2016Article
- Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.Journal of microbiological methods · 2016Article
- Future technology insight: mass spectrometry imaging as a tool in drug research and development.British journal of pharmacology · 2015Review
- LPS modification promotes maintenance of Yersinia pestis in fleas.Microbiology (Reading, England) · 2015Article
- Activation of innate immune responses by Haemophilus influenzae lipooligosaccharide.Clinical and vaccine immunology : CVI · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely used for structural characterization of bacterial endotoxins (lipid A). However, the mass spectrometric behavior of the lipid A molecule is highly dependent on the matrix. Furthermore, this dependence is strongly linked to phosphorylation patterns. Using lipid A from Escherichia coli O116 as a model system, we have investigated the effects of different matrices and comatrix compounds on the analysis of lipid A. In this paper, we report a highly sensitive matrix system for lipid A analysis, which consists of 5-chloro-2-mercaptobenzothiazole matrix and EDTA ammonium salt comatrix. This matrix system enhances the sensitivity of the analysis of diphosphorylated lipid A species by more than 100-fold and in addition provides tolerance to high concentrations of sodium dodecyl sulfate (SDS) and tolerance to sodium chloride and calcium chloride at 10 muM, 100 muM, and 10 muM concentrations. The method was further evaluated for analysis of lipid A species with different phosphorylation patterns and from different bacteria, including Helicobacter pylori, Salmonella enterica serovar Riogrande, and Francisella novicida.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.