ArticleJournal of bacteriology2010
SpoIID-mediated peptidoglycan degradation is required throughout engulfment during Bacillus subtilis sporulation.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 53 citations in OpenAlex.
- Distinctive spore architecture and developmental biology ofJournal of bacteriology · 2026Article
- Identification of sporulation genes in Bacillus anthracis highlights similarities and significant differences with Bacillus subtilis.PLoS biology · 2025Article
- Convergent genomic responses of human gut bacteria to variations in industrialization.bioRxiv : the preprint server for biology · 2025Article
- Developmentally regulated proteolysis by MdfA and ClpCP mediates metabolic differentiation duringGenes & development · 2025Article
- Ribosomes translocation into the spore of Bacillus subtilis is highly organised and requires peptidoglycan rearrangements.Nature communications · 2025Article
- Acetylation of the histone-like protein HBsu at specific sites alters gene expression during sporulation inFrontiers in microbiology · 2025Article
- Time-Course Transcriptome Analysis ofMicroorganisms · 2023Article
- Article
- Role of SpoIVA ATPase Motifs during Clostridioides difficile Sporulation.Journal of bacteriology · 2020Article
- Shaping an Endospore: Architectural Transformations DuringAnnual review of microbiology · 2020Review
- Article
- From Root to Tips: Sporulation Evolution and Specialization in Bacillus subtilis and the Intestinal Pathogen Clostridioides difficile.Molecular biology and evolution · 2019Article
- Sporulation and Germination in Clostridial Pathogens.Microbiology spectrum · 2019Review
- Article
- The molecular architecture of engulfment duringeLife · 2019Article
- Differential requirements for conserved peptidoglycan remodeling enzymes during Clostridioides difficile spore formation.Molecular microbiology · 2018Article
- Efficient production of extracellular pullulanase in Bacillus subtilis ATCC6051 using the host strain construction and promoter optimization expression system.Microbial cell factories · 2018Article
- Lytic transglycosylases: concinnity in concision of the bacterial cell wall.Critical reviews in biochemistry and molecular biology · 2017Review
- Disruption of Two-component System LytSR Affects Forespore Engulfment inFrontiers in cellular and infection microbiology · 2017Article
- Cell-wall remodeling drives engulfment duringeLife · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
SpoIID is a membrane-anchored enzyme that degrades peptidoglycan and is essential for engulfment and sporulation in Bacillus subtilis. SpoIID is targeted to the sporulation septum, where it interacts with two other proteins required for engulfment: SpoIIP and SpoIIM. We changed conserved amino acids in SpoIID to alanine to determine whether there was a correlation between the effect of each substitution on the in vivo and in vitro activities of SpoIID. We identified one amino acid substitution, E88A, that eliminated peptidoglycan degradation activity and one, D210A, that reduced it, as well as two substitutions that destabilized the protein in B. subtilis (R106A and K203A). Using these mutants, we show that the peptidoglycan degradation activity of SpoIID is required for the first step of engulfment (septal thinning), as well as throughout membrane migration, and we show that SpoIID levels are substantially above the minimum required for engulfment. The inactive mutant E88A shows increased septal localization compared to the wild type, suggesting that the degradation cycle of the SpoIID/SpoIIP complex is accompanied by the activity-dependent release of SpoIID from the complex and subsequent rebinding. This mutant is also capable of moving SpoIIP across the sporulation septum, suggesting that SpoIID binding, but not peptidoglycan degradation activity, is needed for relocalization of SpoIIP. Finally, the mutant with reduced activity (D210A) causes uneven engulfment and time-lapse microscopy indicates that the fastest-moving membrane arm has greater concentrations of SpoIIP than the slower-moving arm, demonstrating a correlation between SpoIIP protein levels and the rate of membrane migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.