ArticleEukaryotic cell2010
Localization and targeting of an unusual pyridine nucleotide transhydrogenase in Entamoeba histolytica.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- A RhoGEF activates RacM to selectively promote motility without affecting macropinocytosis in Entamoeba histolytica.Journal of cell science · 2026Article
- EhRacM differentially regulates macropinocytosis and motility in the enteric protozoan parasite Entamoeba histolytica.PLoS pathogens · 2024Article
- Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity.NPJ biofilms and microbiomes · 2023Article
- The mitosome of the anaerobic parasitic protist Entamoeba histolytica: A peculiar and minimalist mitochondrion-related organelle.The Journal of eukaryotic microbiology · 2022Review
- Article
- Anaerobic derivates of mitochondria and peroxisomes in the free-living amoeba Pelomyxa schiedti revealed by single-cell genomics.BMC biology · 2022Article
- Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasiteFrontiers in cellular and infection microbiology · 2022Article
- Article
- Import ofMicroorganisms · 2020Article
- NAD(P) transhydrogenase has vital non-mitochondrial functions in malaria parasite transmission.EMBO reports · 2020Article
- Two isotypes of phosphatidylinositol 3-phosphate-binding sorting nexins play distinct roles in trogocytosis in Entamoeba histolytica.Cellular microbiology · 2020Article
- Cysteine protease-binding protein family 6 mediates the trafficking of amylases to phagosomes in the enteric protozoan Entamoeba histolytica.Infection and immunity · 2013Article
- The mitochondrion-like organelle of Trimastix pyriformis contains the complete glycine cleavage system.PloS one · 2013Article
- Novel TPR-containing subunit of TOM complex functions as cytosolic receptor for Entamoeba mitosomal transport.Scientific reports · 2013Article
- A novel class of cysteine protease receptors that mediate lysosomal transport.Cellular microbiology · 2012Article
- Novel transmembrane receptor involved in phagosome transport of lysozymes and β-hexosaminidase in the enteric protozoan Entamoeba histolytica.PLoS pathogens · 2012Article
- Dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite Entamoeba histolytica.PLoS neglected tropical diseases · 2012Article
- Sulfate activation in mitosomes plays an important role in the proliferation of Entamoeba histolytica.PLoS neglected tropical diseases · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyridine nucleotide transhydrogenase (PNT) catalyzes the direct transfer of a hydride-ion equivalent between NAD(H) and NADP(H) in bacteria and the mitochondria of eukaryotes. PNT was previously postulated to be localized to the highly divergent mitochondrion-related organelle, the mitosome, in the anaerobic/microaerophilic protozoan parasite Entamoeba histolytica based on the potential mitochondrion-targeting signal. However, our previous proteomic study of isolated phagosomes suggested that PNT is localized to organelles other than mitosomes. An immunofluorescence assay using anti-E. histolytica PNT (EhPNT) antibody raised against the NADH-binding domain showed a distribution to the membrane of numerous vesicles/vacuoles, including lysosomes and phagosomes. The domain(s) required for the trafficking of PNT to vesicles/vacuoles was examined by using amoeba transformants expressing a series of carboxyl-terminally truncated PNTs fused with green fluorescent protein or a hemagglutinin tag. All truncated PNTs failed to reach vesicles/vacuoles and were retained in the endoplasmic reticulum. These data indicate that the putative targeting signal is not sufficient for the trafficking of PNT to the vesicular/vacuolar compartments and that full-length PNT is necessary for correct transport. PNT displayed a smear of >120 kDa on SDS-PAGE gels. PNGase F and tunicamycin treatment, chemical degradation of carbohydrates, and heat treatment of PNT suggested that the apparent aberrant mobility of PNT is likely attributable to its hydrophobic nature. PNT that is compartmentalized to the acidic compartments is unprecedented in eukaryotes and may possess a unique physiological role in E. histolytica.
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