ArticleThe Journal of biological chemistry2010
Fluorescent n-3 and n-6 very long chain polyunsaturated fatty acids: three-photon imaging in living cells expressing liver fatty acid-binding protein.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Effect of liver fatty acid binding protein (L-FABP) gene ablation on lipid metabolism in high glucose diet (HGD) pair-fed mice.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- Scp-2/Scp-x ablation in Fabp1 null mice differentially impacts hepatic endocannabinoid level depending on dietary fat.Archives of biochemistry and biophysics · 2018Article
- Loss of fatty acid binding protein-1 alters the hepatic endocannabinoid system response to a high-fat diet.Journal of lipid research · 2017Article
- Effect of Fabp1/Scp-2/Scp-x Ablation on Whole Body and Hepatic Phenotype of Phytol-Fed Male Mice.Lipids · 2017Article
- Fabp1 gene ablation inhibits high-fat diet-induced increase in brain endocannabinoids.Journal of neurochemistry · 2017Article
- FABP1: A Novel Hepatic Endocannabinoid and Cannabinoid Binding Protein.Biochemistry · 2016Article
- Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels.Lipids · 2016Article
- FABP-1 gene ablation impacts brain endocannabinoid system in male mice.Journal of neurochemistry · 2016Article
- Review
- Different Fatty Acids Compete with Arachidonic Acid for Binding to the Allosteric or Catalytic Subunits of Cyclooxygenases to Regulate Prostanoid Synthesis.The Journal of biological chemistry · 2016Article
- Structural and functional interaction of fatty acids with human liver fatty acid-binding protein (L-FABP) T94A variant.The FEBS journal · 2014Article
- The human liver fatty acid binding protein T94A variant alters the structure, stability, and interaction with fibrates.Biochemistry · 2013Article
- Omega-3 fatty acids modulate Weibel-Palade body degranulation and actin cytoskeleton rearrangement in PMA-stimulated human umbilical vein endothelial cells.Marine drugs · 2013Article
- High glucose potentiates L-FABP mediated fibrate induction of PPARα in mouse hepatocytes.Biochimica et biophysica acta · 2013Article
- Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.Journal of lipid research · 2013Article
- Liver fatty acid binding protein gene-ablation exacerbates weight gain in high-fat fed female mice.Lipids · 2013Article
- Impact of L-FABP and glucose on polyunsaturated fatty acid induction of PPARα-regulated β-oxidative enzymes.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- DHA-fluorescent probe is sensitive to membrane order and reveals molecular adaptation of DHA in ordered lipid microdomains.The Journal of nutritional biochemistry · 2013Article
- Inhibitors of Fatty Acid Synthesis Induce PPAR α -Regulated Fatty Acid β -Oxidative Genes: Synergistic Roles of L-FABP and Glucose.PPAR research · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Despite the considerable beneficial effects of n-3 and n-6 very long chain polyunsaturated fatty acids (VLC-PUFAs), very little is known about the factors that regulate their uptake and intracellular distribution in living cells. This issue was addressed in cells expressing liver-type fatty acid-binding protein (L-FABP) by real time multiphoton laser scanning microscopy of novel fluorescent VLC-PUFAs containing a conjugated tetraene fluorophore near the carboxyl group and natural methylene-interrupted n-3 or n-6 grouping. The fluorescent VLC-PUFAs mimicked many properties of their native nonfluorescent counterparts, including uptake, distribution, and metabolism in living cells. The unesterified fluorescent VLC-PUFAs distributed either equally in nuclei versus cytoplasm (22-carbon n-3 VLC-PUFA) or preferentially to cytoplasm (20-carbon n-3 and n-6 VLC-PUFAs). L-FABP bound fluorescent VLC-PUFA with affinity and specificity similar to their nonfluorescent natural counterparts. Regarding n-3 and n-6 VLC-PUFA, L-FABP expression enhanced uptake into the cell and cytoplasm, selectively altered the pattern of fluorescent n-6 and n-3 VLC-PUFA distribution in cytoplasm versus nuclei, and preferentially distributed fluorescent VLC-PUFA into nucleoplasm versus nuclear envelope, especially for the 22-carbon n-3 VLC-PUFA, correlating with its high binding by L-FABP. Multiphoton laser scanning microscopy data showed for the first time VLC-PUFA in nuclei of living cells and suggested a model, whereby L-FABP facilitated VLC-PUFA targeting to nuclei by enhancing VLC-PUFA uptake and distribution into the cytoplasm and nucleoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.