Evidence map›Paper›PMID 20381534›Full record

ArticleJournal of virological methods2010

SYBR Green based real-time RT-PCR assay for detection and genotype prediction of bovine noroviruses and assessment of clinical significance in Norway.

Evert Jor, Mette Myrmel, Christine M Jonassen

Abstract read
In one paragraph

Article in Journal of virological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Phylogeny of bovine norovirus in Egypt based on VP2 gene.International journal of veterinary science and medicine · 2018
    Article
  8. Article
  9. Article
  10. Viral enteritis in calves.The Canadian veterinary journal = La revue veterinaire canadienne · 2017
    Review
  11. Molecular detection of bovine Noroviruses in Argentinean dairy calves: Circulation of a tentative new genotype.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2016
    Article
  12. Bovine noroviruses: A missing component of calf diarrhoea diagnosis.Veterinary journal (London, England : 1997) · 2016
    Review
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Evert JorDepartment of Animal Health, National Veterinary Institute, PO Box 750, Sentrum, NO-0106 Oslo, Norway. evert.jor@vetinst.no
Mette Myrmel
Christine M Jonassen

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel SYBR Green based real-time RT-PCR assay for detection of genogroup III bovine noroviruses (BoNoV) was developed and the assay applied to 419 faecal samples from calves with and without diarrhoea. The samples were obtained from 190 Norwegian dairy and beef herds. BoNoV was detected in 49.6% of the samples from 61.1% of the herds indicating that BoNoV is ubiquitous in Norway. The overall prevalence was not significantly different in diarrhoea and non-diarrhoea samples. Analyses of polymerase gene sequences revealed both genotype III/1 and III/2 with genotype III/2 (Newbury2-like) being the most prevalent. Detected capsid sequences were restricted to Newbury2-like and the chimeric Bo/Thirsk10/00/UK strain. The RNA polymerase genotypes of the circulating BoNoVs in Norway were predicted by melting temperature analysis. Additional data from a challenge experiment suggest that a high proportion of young calves are shedding low levels of BoNoV for a prolonged time after recovering from the associated diarrhoea. The findings may explain some of the discrepancies in detection rates from previous studies and explain why some studies have failed to detect significant prevalence differences between calves with and without diarrhoea. It may also shed new light on some epidemiological aspects of norovirus infections.

Indexed as

AnimalsBenzothiazolesCaliciviridae InfectionsCattleCattle DiseasesDiaminesFecesGastroenteritisGenotypeMolecular EpidemiologyMolecular Sequence DataNorovirusNorwayOrganic ChemicalsPrevalenceQuinolinesBenzothiazolesDiaminesOrganic ChemicalsQuinolinesRNA, ViralSYBR Green I

Identifiers

PMID20381534
PMCPMC7112832

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.