ArticleExperimental cell research2010
Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres.
Article in Experimental cell research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- iPSC-derived skeletal muscle spheroids for Duchenne Muscular Dystrophy modeling.Skeletal muscle · 2026Article
- Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue.ACS biomaterials science & engineering · 2024Article
- Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies.Frontiers in bioengineering and biotechnology · 2023Article
- Microenergy acoustic pulse therapy restores urethral wall integrity and continence in a rat model of female stress incontinence.Neurourology and urodynamics · 2022Article
- Article
- Available In Vitro Models for Human Satellite Cells from Skeletal Muscle.International journal of molecular sciences · 2021Review
- 3D in vitro models of skeletal muscle: myopshere, myobundle and bioprinted muscle construct.Veterinary research · 2021Review
- Featured characteristics and pivotal roles of satellite cells in skeletal muscle regeneration.Journal of muscle research and cell motility · 2020Article
- Microcarriers for Upscaling Cultured Meat Production.Frontiers in nutrition · 2020Review
- Isolation and characterization of myogenic precursor cells from human cremaster muscle.Scientific reports · 2019Article
- A Three-Dimensional Culture Model of Reversibly Quiescent Myogenic Cells.Stem cells international · 2019Article
- Metformin Delays Satellite Cell Activation and Maintains Quiescence.Stem cells international · 2019Article
- A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues.SLAS technology · 2018Article
- Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three-Dimensional Fibrin Gel.Stem cells translational medicine · 2017Article
- Myospheres are composed of two cell types: one that is myogenic and a second that is mesenchymal.PloS one · 2015Article
- Amino acid residues 489-503 of dihydropyridine receptor (DHPR) β1a subunit are critical for structural communication between the skeletal muscle DHPR complex and type 1 ryanodine receptor.The Journal of biological chemistry · 2014Article
- Derivation of myogenic progenitors directly from human pluripotent stem cells using a sphere-based culture.Stem cells translational medicine · 2014Article
- Isolation and in vitro propagation of human skeletal muscle progenitor cells from fetal muscle.Cell biology international · 2013Article
- Paracrine effects of bone marrow soup restore organ function, regeneration, and repair in salivary glands damaged by irradiation.PloS one · 2013Article
- Perivascular cells for regenerative medicine.Journal of cellular and molecular medicine · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The effectiveness of cell-based therapy to treat muscle disease has been hampered by difficulties in isolating, maintaining and propagating the stem cells that are needed for treatment. Here we report the isolation of muscle-derived stem cells from both young and old mice and their propagation over extended periods of time in culture as "free-floating" myospheres. Analysis of these sphere-forming cells showed that they express stem cell antigen-1 (Sca-1), beta1 integrin (CD29), Thy-1 (CD90), and CD34, but did not express CD45, CD31, or myogenic markers (Pax7, Myf5, and MyoD). We found that cells derived from myospheres and then grown adherently (MDACs) behaved similar to primary myoblasts, in that these cells expressed myogenic markers and were able to easily form multinucleated myotubes. Unlike the parental myospheres but analogous to primary myoblasts, MDACs expressed Pax7, Myf5, and MyoD, indicating that the parent myosphere cells were a more primitive type of cell. In support of this we demonstrated that myospheres were also able to differentiate into adipogenic and osteogenic cells in culture, as well as being able to contribute to injured muscle in vivo. In summary, we report that primitive adult muscle stem cells can be easily isolated and sustained in culture as myospheres.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.