ArticlePlant methods2010
Protocol: fine-tuning of a Chromatin Immunoprecipitation (ChIP) protocol in tomato.
Article in Plant methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Protocols for Chromatin Immunoprecipitation Sequencing and qPCR to Investigate Pytohormone-Responsive Transcriptional Regulation in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Chromatin Immunoprecipitation in Crops.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Differential epigenetic regulation by blue and UV-A light reveals the key role of CsSDG36-mediated H3K4 methylation in leaf development and secondary metabolism in Camellia sinensis.Genome biology · 2025Article
- H3K4me3 changes occur in cell wall genes during the development ofFrontiers in plant science · 2024Article
- Self-grafting-induced epigenetic changes leading to drought stress tolerance in tomato plants.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
- Systematic evaluation of chromatin immunoprecipitation sequencing to study histone occupancy in dormancy transitions of grapevine buds.Tree physiology · 2023Article
- An optimized ChIP-Seq framework for profiling histone modifications inPlant direct · 2022Article
- Genome-Wide Identification of theFrontiers in plant science · 2021Article
- An optimised chromatin immunoprecipitation (ChIP) method for starchy leaves of Nicotiana benthamiana to study histone modifications of an allotetraploid plant.Molecular biology reports · 2020Article
- A native chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in strawberry fruits.Plant methods · 2020Article
- The chromatin remodeler ZmCHB101 impacts alternative splicing contexts in response to osmotic stress.Plant cell reports · 2019Article
- The chromatin remodeler ZmCHB101 impacts expression of osmotic stress-responsive genes in maize.Plant molecular biology · 2018Article
- Article
- Manipulation of Light Signal Transduction Factors as a Means of Modifying Steroidal Glycoalkaloids Accumulation in Tomato Leaves.Frontiers in plant science · 2018Article
- Epigenetic regulation of the expression of WRKY75 transcription factor in response to biotic and abiotic stresses in Solanaceae plants.Plant cell reports · 2018Article
- Determination of histone epigenetic marks in Arabidopsis and tomato genes in the early response to Botrytis cinerea.Plant cell reports · 2018Article
- Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana.Journal of experimental botany · 2016Article
- Protein-protein and DNA-protein interactions mediate induction of defense genes by fruit extract of Azadirachta indica A. Juss. in Solanum lycopersicum L.Plant cell reports · 2015Article
- Enhanced oxidative stress resistance through activation of a zinc deficiency transcription factor in Brachypodium distachyon.Plant physiology · 2014Article
- Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor.BMC plant biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSearching thoroughly for plant cis-elements corresponding to transcription factors is worthwhile to reveal novel gene activation cascades. At the same time, a great deal of research is currently focused on epigenetic events in plants. A widely used method serving both purposes is chromatin immunoprecipitation, which was developed for Arabidopsis and other plants but is not yet operational for tomato (Solanum lycopersicum), a model plant species for a group of economically important crops.
resultsWe developed a chromatin immunoprecipitation protocol suitable for tomato by adjusting the parameters to optimise in vivo crosslinking, purification of nuclei, chromatin extraction, DNA shearing and precipitate analysis using real-time PCR. Results were obtained with two different antibodies, five control loci and two normalisation criteria.
conclusionHere we provide a chromatin immunoprecipitation procedure for tomato leaves that could be combined with high-throughput sequencing to generate a detailed map of epigenetic modifications or genome-wide nucleosome positioning data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.