ArticleCytotherapy2010
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Article in Cytotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it, 123 citations in OpenAlex.
- Systematic review of patient factors affecting adipose stem cell viability and function: implications for regenerative therapy.Stem cell research & therapy · 2017Pooled it
- Effect of cyclic mechanical stimulation on the expression of osteogenesis genes in human intraoral mesenchymal stromal and progenitor cells.BioMed research international · 2014Trial
- Immune-related antigens, surface molecules and regulatory factors in human-derived mesenchymal stromal cells: the expression and impact of inflammatory priming.Stem cell reviews and reports · 2012Trial
- Optimization of fat grafting to attenuate radiation damage: a narrative review.Gland surgery · 2026Review
- Toward Efficient Beige Adipogenesis: Protocol Optimization Using Adipose-Derived Stem Cells.Cells · 2025Article
- Understanding and controlling the variables for stromal vascular fraction therapy.World journal of stem cells · 2024Review
- The microalgaMaterials today. Bio · 2024Article
- Adipose-derived stem cell-based optimization strategies for musculoskeletal regeneration: recent advances and perspectives.Stem cell research & therapy · 2024Review
- Myogenic Differentiation and Immunomodulatory Properties of Rat Adipose-Derived Mesenchymal Stem/Stromal Cells.Biology · 2024Article
- Cartilage lesion size and number of stromal vascular fraction (SVF) cells strongly influenced the SVF implantation outcomes in patients with knee osteoarthritis.Journal of experimental orthopaedics · 2023Article
- Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation.Frontiers in bioengineering and biotechnology · 2023Article
- Specific features of ex-obese patients significantly influence the functional cell properties of adipose-derived stromal cells.Journal of cellular and molecular medicine · 2022Article
- Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review).Biomedicines · 2022Review
- Optimization of Mesenchymal Stromal Cell (MSC) Manufacturing Processes for a Better Therapeutic Outcome.Frontiers in immunology · 2022Review
- Human adipose-derived stromal/stem cells expressing doublecortin improve cartilage repair in rabbits and monkeys.NPJ Regenerative medicine · 2021Article
- Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method.Cells · 2021Article
- Effect of Breast Cancer and Adjuvant Therapy on Adipose-Derived Stromal Cells: Implications for the Role of ADSCs in Regenerative Strategies for Breast Reconstruction.Stem cell reviews and reports · 2021Review
- Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.Journal of stem cells & regenerative medicine · 2021Article
- Fat-On-A-Chip Models for Research and Discovery in Obesity and Its Metabolic Comorbidities.Tissue engineering. Part B, Reviews · 2020Review
- Safety and Efficacy of Human Adipose-Derived Stromal/Stem Cell Therapy in an Immunocompetent Murine Pressure Ulcer Model.Stem cells and development · 2020Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
BACKGROUND
aimsAdipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yields from human subcutaneous lipoaspirates. This study reports our recent experience of isolating and immunophenotypically characterizing ASC from >60 human patients with a mean age of 43.6 and body mass index (BMI) of 27.
methodsWe examined the ASC yield per unit volume of lipoaspirate tissue, the surface antigen profile based on flow cytometry, histochemical differentiation potential along the adipogenic and osteogenic pathways, and expression of adipogenic mRNA by transcriptomic microarray and reverse transcription (RT)-polymerase chain reaction (PCR).
resultsThe population (n = 64) of predominantly Caucasian (84.3%) female (90.6%) donors had a mean age of 43.6 +/- 11.1 years and a mean BMI of 27.0 +/- 3.8. A yield of 375 +/- 142 x 10(3) ASC was obtained per milliliter of lipoaspirate within a 4.1 +/- 0.7-day culture period (n = 62). The ASC population was uniformly CD29(+) CD34(+) CD44(lo) CD45(lo) CD73(+) CD90(+) CD105(+) and capable of undergoing both adipogenesis and osteogenesis in vitro based on Oil Red O and Alizarin Red staining, respectively. Adipogenic differentiation was associated with a significant induction of multiple mRNA associated with lipid storage and synthesis based on microarray analysis of n = 3 donors. During an adipogenic differentiation time-course, representative mRNA (adiponectin, C/EBPalpha, leptin and LPL) displayed increases of several orders of magnitude.
conclusionsThese findings demonstrate the reproducibility of subcutaneous lipoaspirates as a consistent and abundant source of functional ASC from donors across a spectrum of ages and BMI. These results have relevance for regenerative medical applications exploiting autologous and allogeneic ASC for soft and hard tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.