ArticleJournal of the American Chemical Society2010
Suborganelle sensing of mitochondrial cAMP-dependent protein kinase activity.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 71 citations in OpenAlex.
- A Triangulenum-Based Biosensor Platform for Mapping Kinase Activity in T Cells.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Advancements in mitochondrial-targeted nanotherapeutics: overcoming biological obstacles and optimizing drug delivery.Frontiers in immunology · 2024Review
- Biosensors with Metal Ion-Phosphate Chelation Interaction for Molecular Recognition.Molecules (Basel, Switzerland) · 2023Review
- Abnormal Cyclic Nucleotide Signaling at the Outer Mitochondrial Membrane In Sympathetic Neurons During the Early Stages of Hypertension.Hypertension (Dallas, Tex. : 1979) · 2022Article
- Contribution of Mitochondria to Insulin Secretion by Various Secretagogues.Antioxidants & redox signaling · 2022Review
- The Pancreatic β-Cell: The Perfect Redox System.Antioxidants (Basel, Switzerland) · 2021Review
- A MedChem toolbox for cereblon-directed PROTACs.MedChemComm · 2019Article
- Functional Significance of the Adcy10-Dependent Intracellular cAMP Compartments.Journal of cardiovascular development and disease · 2018Review
- Mitochondrial cAMP signaling.Cellular and molecular life sciences : CMLS · 2016Review
- Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.Proteomes · 2015Review
- Role of soluble adenylyl cyclase in mitochondria.Biochimica et biophysica acta · 2014Review
- Long-wavelength fluorescent reporters for monitoring protein kinase activity.Angewandte Chemie (International ed. in English) · 2014Article
- The inner and outer compartments of mitochondria are sites of distinct cAMP/PKA signaling dynamics.The Journal of cell biology · 2013Article
- A broad spectrum dark quencher: construction of multiple colour protease and photolytic sensors.Chemical communications (Cambridge, England) · 2013Article
- Probes of the mitochondrial cAMP-dependent protein kinase.Biochimica et biophysica acta · 2013Article
- cAMP and mitochondria.Physiology (Bethesda, Md.) · 2013Review
- Monitoring of protein arginine deiminase activity by using fluorescence quenching: multicolor visualization of citrullination.Angewandte Chemie (International ed. in English) · 2013Article
- Phosphorylation of human TFAM in mitochondria impairs DNA binding and promotes degradation by the AAA+ Lon protease.Molecular cell · 2013Article
- Oxidative stress induced mitochondrial protein kinase A mediates cytochrome c oxidase dysfunction.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A fluorescent sensor of protein kinase activity has been developed and used to characterize the compartmentalized location of cAMP-dependent protein kinase activity in mitochondria. The sensor functions via a phosphorylation-induced release of a quencher from a peptide-based substrate, producing a 150-fold enhancement in fluorescence. The quenching phenomenon transpires via interaction of the quencher with Arg residues positioned on the peptide substrate. Although the cAMP-dependent protein kinase is known to be present in mitochondria, the relative amount of enzyme positioned in the major compartments (outer membrane, intermembrane space, and the matrix) of the organelle is unclear. The fluorescent sensor developed in this study was used to reveal the relative matrix/intermembrane space/outer membrane (85:6:9) distribution of PKA in bovine heart mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.