ArticleAnalytical chemistry2010
Identification of immobile single molecules using polarization-modulated asynchronous time delay and integration-mode scanning.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.Nature communications · 2025Article
- Single Molecule Fluorescence Microscopy and Machine Learning for Rhesus D Antigen Classification.Scientific reports · 2016Article
- Streptavidin functionalized polymer nanodots fabricated by visible light lithography.Journal of nanobiotechnology · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
We report the development of a data acquisition method for identifying single molecules on large surfaces with simultaneous characterization of their absorption dipole. The method is based on a previously described device for microarray readout at single molecule sensitivity (Hesse, J.; Sonnleitner, M.; Sonnleitner, A.; Freudenthaler, G.; Jacak, J.; Höglinger, O.; Schindler, H.; Schütz, G. J. Anal. Chem. 2004, 76, 5960-5964). Here, we introduced asynchronous time delay and integration- (TDI-) mode imaging to record also the time course of fluorescence signals: the images thus contain both spatial and temporal information. We demonstrate the principle by modulating the signals via rotating excitation polarization, which allows for discriminating static absorption dipoles against multiple or freely rotating single absorption dipoles. Experiments on BSA carrying different numbers of fluorophores demonstrate the feasibility of the method. Protein species with an average labeling degree of 0.55 and 2.89 fluorophores per protein can be readily distinguished.
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