ArticlePlanta2010
Ice recrystallization inhibition proteins of perennial ryegrass enhance freezing tolerance.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- A DUF3494 ice-binding protein with a root cap domain in a streptophyte glacier ice alga.Frontiers in plant science · 2023Article
- Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms.Life (Basel, Switzerland) · 2022Review
- Association mapping of autumn-seeded rye (Secale cereale L.) reveals genetic linkages between genes controlling winter hardiness and plant development.Scientific reports · 2022Article
- Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis.Frontiers in plant science · 2022Article
- Cold adaptation strategies in plants-An emerging role of epigenetics and antifreeze proteins to engineer cold resilient plants.Frontiers in genetics · 2022Review
- Genetic loci associated with winter survivorship in diverse lowland switchgrass populations.The plant genome · 2021Article
- Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.Biomolecules · 2020Review
- Evolution of Cold Acclimation and Its Role in Niche Transition in the Temperate Grass Subfamily Pooideae.Plant physiology · 2019Article
- Both cold and sub-zero acclimation induce cell wall modification and changes in the extracellular proteome in Arabidopsis thaliana.Scientific reports · 2019Article
- Impact of postharvest exogenous γ-aminobutyric acid treatment on cucumber fruit in response to chilling tolerance.Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Ice-Binding Proteins in Plants.Frontiers in plant science · 2017Review
- Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana.Plant biotechnology journal · 2017Article
- Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize.Frontiers in plant science · 2016Article
- Knockdown of Ice-Binding Proteins in Brachypodium distachyon Demonstrates Their Role in Freeze Protection.PloS one · 2016Article
- Vernalization Requirement and the Chromosomal VRN1-Region can Affect Freezing Tolerance and Expression of Cold-Regulated Genes in Festuca pratensis.Frontiers in plant science · 2016Article
- Antifreeze proteins enable plants to survive in freezing conditions.Journal of biosciences · 2014Review
- Article
- Evidence for adaptive evolution of low-temperature stress response genes in a Pooideae grass ancestor.The New phytologist · 2013Article
- Adaptation to seasonality and the winter freeze.Frontiers in plant science · 2013Article
- Comparative analyses reveal potential uses of Brachypodium distachyon as a model for cold stress responses in temperate grasses.BMC plant biology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ice recrystallization inhibition (IRI) proteins are thought to play an important role in conferring freezing tolerance in plants. Two genes encoding IRI proteins, LpIRI-a and LpIRI-b, were isolated from a relatively cold-tolerant perennial ryegrass cv. Caddyshack. Amino acid alignments among the IRI proteins revealed the presence of conserved repetitive IRI-domain motifs (NxVxxG/NxVxG) in both proteins. Quantitative reverse transcriptase PCR (qRT-PCR) analysis indicated that LpIRI-a was up-regulated approximately 40-fold while LpIRI-b was up-regulated sevenfold after just 1 h of cold acclimation, and by 7 days of cold acclimation the transcripts had increased 8,000-fold for LpIRI-a and 1,000-fold for LpIRI-b. Overexpression of either LpIRI-a or LpIRI-b gene in Arabidopsis increased survival rates of the seedlings following a freezing test under both cold-acclimated and nonacclimated conditions. For example, without cold acclimation a -4 degrees C treatment reduced the wild type's survival rate to an average of 73%, but resulted in survival rates of 85-100% for four transgenic lines. With cold acclimation, a -12 degrees C treatment reduced the wild type's survival rate to an average of 38.7%, while it resulted in a survival rate of 51-78.5% for transgenic lines. After cold acclimation, transgenic Arabidopsis plants overexpressing either LpIRI-a or LpIRI-b gene exhibited a consistent reduction in freezing-induced ion leakage at -8, -9, and -10 degrees C. Furthermore, the induced expression of the LpIRI-a and LpIRI-b proteins in transgenic E. coli enhanced the freezing tolerance in host cells. Our results suggest that IRI proteins play an important role in freezing tolerance in plants.
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