ArticleNature protocols2010
Generating heparan sulfate saccharide libraries for glycomics applications.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Selective Binding of Heparin/Heparan Sulfate Oligosaccharides to Factor H and Factor H-Related Proteins: Therapeutic Potential for C3 Glomerulopathies.Frontiers in immunology · 2021Article
- By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans.Scientific reports · 2019Article
- Enhancing the glycosciences toolkit: new GAGs in the lineup.Nature methods · 2018Article
- Immobilization alters heparin cleaving properties of heparinase I.Glycobiology · 2017Article
- Glycosaminoglycanomics: where we are.Glycoconjugate journal · 2017Review
- Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors.Molecules (Basel, Switzerland) · 2017Article
- Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity.Molecular bioSystems · 2016Article
- Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1.Journal of the American Chemical Society · 2016Article
- Glycoarray Technologies: Deciphering Interactions from Proteins to Live Cell Responses.Microarrays (Basel, Switzerland) · 2016Review
- Heparan sulfate and heparin interactions with proteins.Journal of the Royal Society, Interface · 2015Review
- Article
- The solution structure of heparan sulfate differs from that of heparin: implications for function.The Journal of biological chemistry · 2013Article
- Tunable heparan sulfate mimetics for modulating chemokine activity.Journal of the American Chemical Society · 2013Article
- Chemoenzymatic synthesis of glycosaminoglycans: re-creating, re-modeling and re-designing nature's longest or most complex carbohydrate chains.Glycobiology · 2013Review
- Glycomics approaches for the bioassay and structural analysis of heparin/heparan sulphates.Metabolites · 2012Article
- Diversification of the structural determinants of fibroblast growth factor-heparin interactions: implications for binding specificity.The Journal of biological chemistry · 2012Article
- The solution structure of heparan sulfate differs from that of heparin: implications for function.The Journal of biological chemistry · 2011Article
- An expression system for screening of proteins for glycan and protein interactions.Analytical biochemistry · 2011Article
- Disaccharide compositional analysis of heparan sulfate and heparin polysaccharides using UV or high-sensitivity fluorescence (BODIPY) detection.Nature protocols · 2010Article
- Specific sides to multifaceted glycosaminoglycans are observed in embryonic development.Seminars in cell & developmental biology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Natural and semi-synthetic heparan sulfate (HS) saccharide libraries are a valuable resource for investigating HS structure-function relationships, enabling high-throughput glycomics studies. Owing to the difficulty of chemical or in vitro enzymatic synthesis of HS saccharides, the structural diversity displayed in saccharides from tissue or cell sources cannot be readily accessed. In contrast, saccharide libraries can be generated by partial digestion of tissue-derived HS polysaccharide chains and chromatographic fractionation of the resulting saccharide mixtures. Fractionation is initially on the basis of hydrodynamic volume, using size exclusion chromatography. Further fractionation, on the basis of charge using strong anion exchange, can subsequently be applied. Desalting and sample concentration follows each fractionation step. Chromatographic fractions are generated that contain purified, or partially purified, saccharides. Here we describe a comprehensive protocol for generation of structurally diverse natural saccharide libraries from HS variants that is fast (approximately 3 weeks) and reproducible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.