ArticleNucleic acids research2010
Posttranscriptional repression of the cel gene of the ColE7 operon by the RNA-binding protein CsrA of Escherichia coli.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Nutrients and flow shape the cyclic dominance games betweenPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
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- The Evolution of Mass Cell Suicide in Bacterial Warfare.Current biology : CB · 2020Article
- Dynamics of ColicinE2 production and release determine the competitive success of a toxin-producing bacterial population.Scientific reports · 2020Article
- Article
- Expansion of the SOS regulon of Vibrio cholerae through extensive transcriptome analysis and experimental validation.BMC genomics · 2018Article
- CsrA and its regulators control the time-point of ColicinE2 release in Escherichia coli.Scientific reports · 2018Article
- Article
- Bet-hedging in bacteriocin producing Escherichia coli populations: the single cell perspective.Scientific reports · 2017Article
- Hierarchical Post-transcriptional Regulation of Colicin E2 Expression in Escherichia coli.PLoS computational biology · 2016Article
- Circuitry Linking the Catabolite Repression and Csr Global Regulatory Systems of Escherichia coli.Journal of bacteriology · 2016Article
- Reporters for Single-Cell Analysis of Colicin Ib Expression in Salmonella enterica Serovar Typhimurium.PloS one · 2015Article
- Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon.PloS one · 2015Article
- Nutrient-responsive regulation determines biodiversity in a colicin-mediated bacterial community.BMC biology · 2014Article
- A sequence-based approach for prediction of CsrA/RsmA targets in bacteria with experimental validation in Pseudomonas aeruginosa.Nucleic acids research · 2014Article
- A novel endogenous induction of ColE7 expression in a csrA mutant of Escherichia coli.Current microbiology · 2013Article
- Translational repression of NhaR, a novel pathway for multi-tier regulation of biofilm circuitry by CsrA.Journal of bacteriology · 2012Article
- Carbon storage regulator A (CsrA(Bb)) is a repressor of Borrelia burgdorferi flagellin protein FlaB.Molecular microbiology · 2011Article
- CsrA and TnaB coregulate tryptophanase activity to promote exotoxin-induced killing of Caenorhabditis elegans by enteropathogenic Escherichia coli.Journal of bacteriology · 2011Article
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5 authors at 3 institutions in 2 countries.
Funding
Abstract
Carbon storage regulator (CsrA) is a eubacterial RNA-binding protein that acts as a global regulator of many functionally diverse chromosomal genes. Here, we reveal that CsrA represses expression from an extrachromosomal element of Escherichia coli, the lysis gene (cel) of the ColE7 operon (cea-cei-cel). This operon and colicin expression are activated upon SOS response. Disruption of csrA caused approximately 5-fold increase of the lysis protein. Gel mobility shift assays established that both the single-stranded loop of the T1 stem-loop distal to cei, and the putative CsrA binding site overlapping the Shine-Dalgarno sequence (SD) of the cel gene are important for CsrA binding. Substitution mutations at SD relieved CsrA-dependent repression of the cel gene in vivo. Steady-state levels and half-life of the cel mRNA were not affected by CsrA, implying that regulation is mediated at the translational level. Levels of CsrB and CsrC sRNAs, which bind to and antagonize CsrA, were drastically reduced upon induction of the SOS response, while the CsrA protein itself remained unaffected. Thus, CsrA is a trans-acting modulator that downregulates the expression of lysis protein, which may confer a survival advantage on colicinogenic E. coli under environment stress conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.