ArticleMicrobiology (Reading, England)2010
Use of optical mapping to sort uropathogenic Escherichia coli strains into distinct subgroups.
Article in Microbiology (Reading, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Article
- Cultivation-Free Typing of Bacteria Using Optical DNA Mapping.ACS infectious diseases · 2020Article
- OMTools: a software package for visualizing and processing optical mapping data.Bioinformatics (Oxford, England) · 2017Article
- Noise reduction in single time frame optical DNA maps.PloS one · 2017Article
- Article
- Article
- Comparative Genomics and Characterization of Hybrid Shigatoxigenic and Enterotoxigenic Escherichia coli (STEC/ETEC) Strains.PloS one · 2015Article
- Whole-genome mapping: a new paradigm in strain-typing technology.Journal of clinical microbiology · 2013Review
- Enhanced de novo assembly of high throughput pyrosequencing data using whole genome mapping.PloS one · 2013Article
- Comparative whole-genome mapping to determine Staphylococcus aureus genome size, virulence motifs, and clonality.Journal of clinical microbiology · 2012Article
- Assessment of whole-genome mapping in a well-defined outbreak of Salmonella enterica serotype Saintpaul.Journal of clinical microbiology · 2012Article
- Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes.Journal of industrial microbiology & biotechnology · 2012Article
- Virulence potential and genomic mapping of the worldwide clone Escherichia coli ST131.PloS one · 2012Article
- Escherichia coli pyomyositis in an immunocompromised host.WMJ : official publication of the State Medical Society of Wisconsin · 2011Review
- Article
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11 authors.
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Abstract
Optical maps were generated for 33 uropathogenic Escherichia coli (UPEC) isolates. For individual genomes, the NcoI restriction fragments aligned into a unique chromosome map for each individual isolate, which was then compared with the in silico restriction maps of all of the sequenced E. coli and Shigella strains. All of the UPEC isolates clustered separately from the Shigella strains as well as the laboratory and enterohaemorrhagic E. coli strains. Moreover, the individual strains appeared to cluster into distinct subgroups based on the dendrogram analyses. Phylogenetic grouping of these 33 strains showed that 32/33 were the B2 subgroup and 1/33 was subgroup A. To further characterize the similarities and differences among the 33 isolates, pathogenicity island (PAI), haemolysin and virulence gene comparisons were performed. A strong correlation was observed between individual subgroups and virulence factor genes as well as haemolysis activity. Furthermore, there was considerable conservation of sequenced-strain PAIs in the specific subgroups. Strains with different antibiotic-resistance patterns also appeared to sort into separate subgroups. Thus, the optical maps distinguished the UPEC strains from other E. coli strains and further subdivided the strains into distinct subgroups. This optical mapping procedure holds promise as an alternative way to subgroup all E. coli strains, including those involved in infections outside of the intestinal tract and epidemic strains with distinct patterns of antibiotic resistance.
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