ArticleThe Journal of biological chemistry2010
Dispersed Chromosomal Stat5b-binding elements mediate growth hormone-activated insulin-like growth factor-I gene transcription.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition.eLife · 2024Article
- Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition.bioRxiv : the preprint server for biology · 2023Article
- The Origination of Growth Hormone/Insulin-Like Growth Factor System: A Story From Ancient Basal Chordate Amphioxus.Frontiers in endocrinology · 2022Review
- The GH-IGF-1 Axis in Circadian Rhythm.Frontiers in molecular neuroscience · 2021Review
- Role of Alternatively Spliced Messenger RNA (mRNA) Isoforms of the Insulin-Like Growth Factor 1 (IGF1) in Selected Human Tumors.International journal of molecular sciences · 2020Review
- Regulation of gene expression by growth hormone.Molecular and cellular endocrinology · 2020Review
- Characterizing the complexity of Australian marsupial insulin-like growth factor 1 genes.Molecular and cellular endocrinology · 2019Article
- Growth hormone controls lipolysis by regulation of FSP27 expression.The Journal of endocrinology · 2018Article
- Insulinlike Growth Factor 1 Gene Variation in Vertebrates.Endocrinology · 2018Article
- Epigenetics in Turner syndrome.Clinical epigenetics · 2018Review
- Metabolic, Reproductive, and Neurologic Abnormalities in Agpat1-Null Mice.Endocrinology · 2017Article
- GH/IGF-1 Signaling and Current Knowledge of Epigenetics; a Review and Considerations on Possible Therapeutic Options.International journal of molecular sciences · 2017Review
- Activation of Male Liver Chromatin Accessibility and STAT5-Dependent Gene Transcription by Plasma Growth Hormone Pulses.Endocrinology · 2017Article
- Variation in the Insulin-Like Growth Factor 1 Gene in Primates.Endocrinology · 2017Article
- Perinatal high methyl donor alters gene expression in IGF system in male offspring without altering DNA methylation.Future science OA · 2017Article
- Article
- Defining human insulin-like growth factor I gene regulation.American journal of physiology. Endocrinology and metabolism · 2016Article
- STAT5B deficiency: Impacts on human growth and immunity.Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society · 2016Review
- Intrauterine growth restriction perturbs nucleosome depletion at a growth hormone-responsive element in the mouse IGF-1 gene.Physiological genomics · 2015Article
- Identifying growth hormone-regulated enhancers in the Igf1 locus.Physiological genomics · 2015Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
The growth hormone (GH)-insulin-like growth factor-I (IGF-I) axis regulates somatic growth during childhood and orchestrates tissue repair throughout the life span. Recently described inactivating mutations in Stat5b in humans with impaired growth have focused attention on this transcription factor as a key agent linking GH-stimulated signals to IGF-I gene expression, and several putative Stat5b sites have been identified in the IGF-I gene. Here, we define and characterize potential GH- and Stat5b-activated chromosomal enhancers that can regulate IGF-I gene transcription. Of 89 recognizable Stat5 sequences in 200 kb centering on the rat IGF-I gene, 22 resided within conserved regions and/or were identical among different species. Only 15 of these sites, organized into 7 distinct domains, were found to bind Stat5b by quantitative chromatin immunoprecipitation assays in liver chromatin of rats, but only after acute GH treatment. These sites could bind Stat5b in vitro, and individual domains could mediate GH- and Stat5b-stimulated IGF-I promoter activity in cultured cells. Further analyses revealed that four Stat5b domains possessed chromatin signatures of enhancers, including binding of co-activators p300 and Med1, and RNA polymerase II. These modifications preceded GH-stimulated recruitment of Stat5b, as did lysine 4 monomethylation of histone H3, which was enriched in 6/7 Stat5b-binding elements. In contrast, histone acetylation was induced by GH but was limited to Stat5b binding domains found within the IGF-I transcription unit. We conclude that GH stimulates recruitment of Stat5b to multiple dispersed regions within the igf1 locus, including several with properties consistent with long range transcriptional enhancers that collectively regulate GH-activated IGF-I gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.