ArticleMolecular biology reports2011
Identification of host-defense genes and development of microsatellite markers from ESTs of hard clam Meretrix meretrix.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed.
- Development and validation of genome-wide SSR molecular markers of Tapes dorsatus.Molecular biology reports · 2024Article
- Transcriptome analysis of the differences in gene expression between testis and ovary in green mud crab (Scylla paramamosain).BMC genomics · 2014Article
- Intracellular Cu/Zn superoxide dismutase (Cu/Zn-SOD) from hard clam Meretrix meretrix: its cDNA cloning, mRNA expression and enzyme activity.Molecular biology reports · 2012Article
- Transcriptomic analysis of the clam Meretrix meretrix on different larval stages.Marine biotechnology (New York, N.Y.) · 2012Article
- Identification and expression of a putative LPS-induced TNF-α factor from Asiatic hard clam Meretrix meretrix.Molecular biology reports · 2012Article
- Article
- Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis.Fish physiology and biochemistry · 2011Article
- Characterization of 10 polymorphic microsatellite markers for Mediterranean blue mussel Mytilus galloprovincialis by EST database mining and cross-species amplification.Journal of genetics · 2011Article
- Sixteen polymorphic simple sequence repeat markers from expressed sequence tags of the Chinese Mitten Crab Eriocheir sinensis.International journal of molecular sciences · 2010Article
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Authors and funding
7 authors.
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Abstract
The hard clam Meretrix meretrix is an economically important shellfish in China. However, genomic research on this species is still at early stage, and few genomic resources are available. The objective of the present study was to generate expressed sequence tags (ESTs), and identify host-defense genes and microsatellite markers for M. meretrix. Three cDNA libraries for intestine, mantle and hepatopancreas were constructed using highly efficient SMART (Switching Mechanism At 5' end of the RNA Transcript) method. A total of 3224 random clones were single-pass sequenced from 5'-ends, resulting in 3129 high-quality (>100 bp) ESTs averaging 734 bp. All the ESTs were assembled by software Cap 3, producing 1796 unigenes-1490 singletons and 306 contigs. All the unigenes were compared to the public protein database using tblastx, and 696 (38.8%) were homologues to known genes while the remaining 1100 (61.2%) appeared to be novel sequences. A total of 31 EST clusters were related to immune and defense functions. They included immune recognition receptors, proteases and protease inhibitors, and other immune-related genes. The screening of 1796 unigenes identified 55 (3.1%) microsatellite-containing sequences, with 20 having sufficient flanking sequences for primer design. Polymerase chain reaction amplification was successful for 12 primer pairs and 7 of them showed polymorphic. The EST collection and microsatellite markers obtained in this study provide a useful resource for further gene discovery and population genetic analysis in M. meretrix.
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