ArticleFish physiology and biochemistry2010
Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis).
Article in Fish physiology and biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed.
- Establishment and Characterization of Novel Channa striata Muscle Cell Line for Scaffold-Based Micro-tissue Production.Marine biotechnology (New York, N.Y.) · 2025Article
- Unveiling Gene Expression Dynamics during Early Embryogenesis inLife (Basel, Switzerland) · 2024Article
- Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility.Fish physiology and biochemistry · 2021Article
- Establishment and characterization of a new cell line from the muscle of humpback grouper (Cromileptes altivelis).Fish physiology and biochemistry · 2020Article
- Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).Fish physiology and biochemistry · 2020Article
- Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.).Fish physiology and biochemistry · 2019Article
- Development and Characterization of a New Cell Line from Olive FlounderDevelopment & reproduction · 2018Article
- Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.Fish physiology and biochemistry · 2018Article
- Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus).Fish physiology and biochemistry · 2017Article
- Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus.Fish physiology and biochemistry · 2016Article
- Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis).Dong wu xue yan jiu = Zoological research · 2015Article
- Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale.Fish physiology and biochemistry · 2015Article
- Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis).Fish physiology and biochemistry · 2014Article
- Development and characterization of a cell line WAF from freshwater shark Wallago attu.Molecular biology reports · 2014Article
- Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2012Article
- Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara).Fish physiology and biochemistry · 2012Article
- Development and characterization of a cell line TTCF from endangered mahseer Tor tor (Ham.).Fish physiology and biochemistry · 2012Article
- Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis.International journal of biological sciences · 2011Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new cell line was established from the heart of a cultured marine fish, half smooth tongue sole (Cynoglossus semilaevis), designated as CSH (Cynoglossus semilaevis heart cell line). The CSH cells grow over 400 days in minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) and 2 ng/ml basic fibroblast growth factor (bFGF). The suitable temperature for the cell growth was 24-30°C with the optimum growth at 24°C and a reduced growth at 12 and 30°C. FBS and bFGF concentration were the two important components for CSH cells proliferation. Twenty percent FBS in the medium was found to be the optimum concentration and bFGF promoted the growth of CSH cells. The double time of the cells at 24°C was determined to 73.39 h. Chromosome analysis revealed that 44% of the cells maintained a normal diploid chromosome number (2n=42) in the CSH cells at Passage 58. The fluorescent signals were observed in CSH after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. CSH cells showed the cytopathic effect (CPE) after infection with lymphosystis disease virus (LCDV). Moreover, the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy, and a segment of MCP gene for major capsid protein of LCDV was found by PCR amplification DNA of virus-infected cells.
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Registered trials
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