ArticlePloS one2010
Targeting of de novo DNA methylation throughout the Oct-4 gene regulatory region in differentiating embryonic stem cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 71 citations in OpenAlex.
- Phylo-Epigenetic Conservation and CpG Erosion inGenes · 2025Article
- Breast cancer cell resistance to hormonal and targeted therapeutics is correlated with the inactivation of the NR6A1 axis.Cancer drug resistance (Alhambra, Calif.) · 2024Article
- The sound of silence: Transgene silencing in mammalian cell engineering.Cell systems · 2022Review
- A mathematical model exhibiting the effect of DNA methylation on the stability boundary in cell-fate networks.Epigenetics · 2021Article
- Organization of the Pluripotent Genome.Cold Spring Harbor perspectives in biology · 2021Review
- Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers.Cell reports · 2020Article
- Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system.Scientific reports · 2019Article
- DNMT3L facilitates DNA methylation partly by maintaining DNMT3A stability in mouse embryonic stem cells.Nucleic acids research · 2019Article
- Article
- Specific or not specific recruitment of DNMTs for DNA methylation, an epigenetic dilemma.Clinical epigenetics · 2018Review
- Article
- Tethering of Lsh at the Oct4 locus promotes gene repression associated with epigenetic changes.Epigenetics · 2018Article
- A refined DNA methylation detection method using MspJI coupled quantitative PCR.Analytical biochemistry · 2017Article
- Deciphering the Epigenetic Code in Embryonic and Dental Pulp Stem Cells.The Yale journal of biology and medicine · 2016Review
- An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation.Nucleic acids research · 2016Article
- Epigenetics in preimplantation mammalian development.Theriogenology · 2016Review
- Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective.Nature medicine · 2016Article
- EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos.Genome research · 2016Article
- Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion.Molecular and cellular biology · 2015Article
- Do all roads lead to Oct4? the emerging concepts of induced pluripotency.Trends in cell biology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Differentiation of embryonic stem (ES) cells is accompanied by silencing of the Oct-4 gene and de novo DNA methylation of its regulatory region. Previous studies have focused on the requirements for promoter region methylation. We therefore undertook to analyse the progression of DNA methylation of the approximately 2000 base pair regulatory region of Oct-4 in ES cells that are wildtype or deficient for key proteins. We find that de novo methylation is initially seeded at two discrete sites, the proximal enhancer and distal promoter, spreading later to neighboring regions, including the remainder of the promoter. De novo methyltransferases Dnmt3a and Dnmt3b cooperate in the initial targeted stage of de novo methylation. Efficient completion of the pattern requires Dnmt3a and Dnmt1, but not Dnmt3b. Methylation of the Oct-4 promoter depends on the histone H3 lysine 9 methyltransferase G9a, as shown previously, but CpG methylation throughout most of the regulatory region accumulates even in the absence of G9a. Analysis of the Oct-4 regulatory domain as a whole has allowed us to detect targeted de novo methylation and to refine our understanding the roles of key protein components in this process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.