ArticleGene therapy2010
Duration and level of transgene expression after gene electrotransfer to skin in mice.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Electroporation in Cancer Therapy: A Simplified Model Derived from the Hodgkin-Huxley Model.Bioelectricity · 2024Article
- Electrochemotherapy Plus IL-2+IL-12 Gene Electrotransfer in Spontaneous Inoperable Stage III-IV Canine Oral Malignant Melanoma.Vaccines · 2023Article
- Moderate Heat-Assisted Gene Electrotransfer as a Potential Delivery Approach for Protein Replacement Therapy through the Skin.Pharmaceutics · 2021Article
- Long-Term Safety and Tolerability of BMP7 and HGF Gene Overexpression in Rabbit Cornea.Translational vision science & technology · 2021Article
- Electroporation-delivered transdermal neostigmine in rats: equivalent action to intravenous administration.Drug design, development and therapy · 2016Article
- Electrotransfection and lipofection show comparable efficiency for in vitro gene delivery of primary human myoblasts.The Journal of membrane biology · 2015Article
- A Comparison of Red Fluorescent Proteins to Model DNA Vaccine Expression by Whole Animal In Vivo Imaging.PloS one · 2015Article
- Evaluation of immunogen delivery by DNA immunization using non-invasive bioluminescence imaging.Human vaccines & immunotherapeutics · 2013Article
- Minicircle DNA is superior to plasmid DNA in eliciting antigen-specific CD8+ T-cell responses.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- What you always needed to know about electroporation based DNA vaccines.Human vaccines & immunotherapeutics · 2012Review
- A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines.Genetic vaccines and therapy · 2012Article
- Nonviral gene delivery of erythropoietin by mesenchymal stromal cells.Gene therapy · 2012Article
- Comparison of plasmid vaccine immunization schedules using intradermal in vivo electroporation.Clinical and vaccine immunology : CVI · 2011Article
- Electro-gene transfer to skin using a noninvasive multielectrode array.Journal of controlled release : official journal of the Controlled Release Society · 2011Article
- Numerical optimization of gene electrotransfer into muscle tissue.Biomedical engineering online · 2010Article
- Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice.Gene therapy · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In development of novel vaccines, attention is drawn to DNA vaccinations. They are heat stable and can be easily produced. Gene electrotransfer is a simple and nonviral means of transferring DNA to cells and tissues and is attracting increasing interest. One very interesting perspective with gene electrotransfer is that choice of tissue can determine the duration of transgene expression. With gene electrotransfer to muscle, long-term expression, that is beyond 1 year, can be obtained, whereas gene electrotransfer to skin gives short-term expression, which is desirable in, for example, DNA vaccinations. Level and duration of transgene expression after gene electrotransfer to skin is essential and here we present data from two independent quantitative studies. Using in vivo bioimaging of a far-red fluorescent molecule, Katushka, allowing for continuous monitoring of local gene expression, compared with measurements of a systemic transgene, that is, serum erythropoietin (EPO) after gene electrotransfer with EPO to skin, we found a significant increase in transgene expression (P< 0.01) with a peak 9 days (Katushka) and 14 days (EPO) after transfection. Duration of expression could be 3-4 weeks, which is a suitable time frame for vaccinations and is applicable, for example, in gene therapy for wound healing or treatment of cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.