ArticleInvestigative ophthalmology & visual science2010
The RIM101 signal transduction pathway regulates Candida albicans virulence during experimental keratomycosis.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Temporal transcriptional response ofeLife · 2024Article
- Multi-Omics Profiling ofMicroorganisms · 2023Article
- Cyclic strain of poly (methyl methacrylate) surfaces triggered the pathogenicity of Candida albicans.Acta biomaterialia · 2023Article
- The Role of Glycoside Hydrolases in S. gordonii and C. albicans Interactions.Applied and environmental microbiology · 2022Article
- Functional diversity in the pH signaling pathway: an overview of the pathway regulation in Neurospora crassa.Current genetics · 2018Review
- Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling.BMC genomics · 2016Article
- Candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes.Molecular oral microbiology · 2015Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
- Robustness analysis on interspecies interaction network for iron and glucose competition between Candida albicans and zebrafish during infection.BMC systems biology · 2014Article
- Profiling of Candida albicans gene expression during intra-abdominal candidiasis identifies biologic processes involved in pathogenesis.The Journal of infectious diseases · 2013Article
- Candida albicans pathogenicity mechanisms.Virulence · 2013Review
- The β-arrestin-like protein Rim8 is hyperphosphorylated and complexes with Rim21 and Rim101 to promote adaptation to neutral-alkaline pH.Eukaryotic cell · 2012Article
- Cryptococcal titan cell formation is regulated by G-protein signaling in response to multiple stimuli.Eukaryotic cell · 2011Article
- Regulatory circuitry governing fungal development, drug resistance, and disease.Microbiology and molecular biology reviews : MMBR · 2011Review
- A fungal pH-responsive signaling pathway regulating Aspergillus adaptation and invasion into the cornea.Investigative ophthalmology & visual science · 2010Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo examine the role of the fungal RIM101 signal transduction pathway in the pathogenesis of Candida albicans keratitis.
methodsC. albicans wild-type strain SC5314, prototrophic mutant control DAY185, and homozygous fungal mutants for the rim8, rim13, rim20, rim101, and phr1 genes were evaluated in vitro using proliferation and filamentation assays. Scarified corneas of BALB/c and C57BL/6J mice were topically inoculated and observed daily for keratitis severity. Corneal adaptation and pathogenicity were assessed ex vivo by maintaining infected porcine corneas for 3 days in an explantation culture system for histologic evaluation of hyphal penetration.
resultsAll C. albicans strains had similar growth kinetics, and SC5314 and DAY185 demonstrated pH-induced filamentation. Fungal mutants had reduced hyphal formation at alkaline and neutral pH, but normal acidic assays ascertained that mutant strains did not have a generalized filamentation defect. SC5314 and DAY185 caused moderate to severe keratitis in mice, whereas fungal strains lacking constituents of the RIM101 pathway had significantly (P<0.05) attenuated severity in vivo. Three days after inoculation of porcine corneas, SC5314 and DAY185 produced hyphae that penetrated 28% and 25%, respectively, of the corneal thickness, and all five mutant strains showed significantly (P<0.05) less stromal penetration.
conclusionsThe RIM101 signal transduction pathway plays an important role in the development of C. albicans keratitis. The fungal pathway intermediates Rim8p, Rim13p, Rim20p, and Rim101p and the downstream cell-wall protein Phr1p are pivotal in the process of corneal invasion by C. albicans.
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