ArticleInvestigative ophthalmology & visual science2010
Pathway-focused arrays reveal increased matrix metalloproteinase-7 (matrilysin) transcription in trachomatous trichiasis.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Rates and risk factors for unfavorable outcomes 6 weeks after trichiasis surgery.Investigative ophthalmology & visual science · 2011Trial
- The conjunctival transcriptome in Ethiopians after trichiasis surgery: associations with the development of eyelid contour abnormalities and the effect of oral doxycycline treatment.Wellcome open research · 2019Article
- Conjunctival Microbiome-Host Responses Are Associated With Impaired Epithelial Cell Health in Both Early and Late Stages of Trachoma.Frontiers in cellular and infection microbiology · 2019Article
- Evaluation of a Chlamydia trachomatis-specific, commercial, real-time PCR for use with ocular swabs.Parasites & vectors · 2018Article
- miRNAs that associate with conjunctival inflammation and ocular Chlamydia trachomatis infection do not predict progressive disease.Pathogens and disease · 2017Article
- The 2016 Bowman Lecture Conjunctival curses: scarring conjunctivitis 30 years on.Eye (London, England) · 2017Review
- Immunofibrogenic Gene Expression Patterns in Tanzanian Children with OcularFrontiers in cellular and infection microbiology · 2017Article
- Increased Epithelial Expression of CTGF and S100A7 with Elevated Subepithelial Expression of IL-1β in Trachomatous Trichiasis.PLoS neglected tropical diseases · 2016Article
- Trachoma and Ocular Chlamydial Infection in the Era of Genomics.Mediators of inflammation · 2015Review
- Risk factors for active trachoma and ocular Chlamydia trachomatis infection in treatment-naïve trachoma-hyperendemic communities of the Bijagós Archipelago, Guinea Bissau.PLoS neglected tropical diseases · 2014Article
- Review
- The conjunctival microbiome in health and trachomatous disease: a case control study.Genome medicine · 2014Article
- Doxycycline prevents matrix remodeling and contraction by trichiasis-derived conjunctival fibroblasts.Investigative ophthalmology & visual science · 2013Article
- Trachoma: protective and pathogenic ocular immune responses to Chlamydia trachomatis.PLoS neglected tropical diseases · 2013Review
- Genome-wide identification of Chlamydia trachomatis antigens associated with trachomatous trichiasis.Investigative ophthalmology & visual science · 2012Article
- Innate immune responses and modified extracellular matrix regulation characterize bacterial infection and cellular/connective tissue changes in scarring trachoma.Infection and immunity · 2012Article
- Active trachoma is associated with increased conjunctival expression of IL17A and profibrotic cytokines.Infection and immunity · 2011Article
- What is causing active trachoma? The role of nonchlamydial bacterial pathogens in a low prevalence setting.Investigative ophthalmology & visual science · 2011Article
- Systemic effector and regulatory immune responses to chlamydial antigens in trachomatous trichiasis.Frontiers in microbiology · 2011Article
- Conjunctival transcriptome in scarring trachoma.Infection and immunity · 2011Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
purposeSeveral genes that are associated with protection from or susceptibility to trachomatous trichiasis (TT) have been identified through genetic association studies. Yet there have been few studies in which gene expression profiles were assessed in TT cases and disease-free controls. The purpose was to identify genes that are differentially expressed in the upper tarsal conjunctiva of subjects with TT.
methodPathway-focused gene arrays were used to screen conjunctival RNA expression of 226 gene transcripts of interest. The screening was followed by validation of differentially expressed genes by qRT-PCR on an independent set of samples. Three different techniques were then used to test for quantitative differences in the recovered conjunctival protein fraction.
resultsFocused arrays identified a set of 13 differentially expressed genes. Validation by qRT-PCR confirmed differential expression in four of these genes (COL1A1, COL7A1, MMP7, and TLR6). Increased expression of MMP7 was the only consistent differentially regulated gene in the conjunctival samples of trichiasis subjects. MMP7 was present in isolated conjunctival proteins and in the tissue culture supernatants of peripheral blood lymphocytes after stimulation.
conclusionsThere is an imbalance in extracellular matrix turnover with minimal contribution of adaptive immune responses at this stage of trichiasis. There was little evidence of broad differential expression in genes characteristic of polar responses of adaptive T cells or macrophages. The control of the MMP7 response and its activity appears significant in the fibrotic changes observed in TT.
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